Detailed information of TA system TA184315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-Xre
• Location: 1019382..1020314
• Replicon: plasmid pSymB
• Accession: NZ_CP066358
• Organism: Sinorhizobium meliloti strain AK76

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: JFX10_RS04505
• Protein ID: WP_198868371.1
• Coordinates: 1019382..1019936 (-)
• Length: 185 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: H0G7T7
• Locus tag: JFX10_RS04510
• Protein ID: WP_003534594.1
• Coordinates: 1019943..1020314 (-)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX10_RS04485	1014937..1016307	+	1371	WP_198868370.1	FAD-binding oxidoreductase	-
JFX10_RS04490	1016392..1016881	-	490	Protein_894	IS630-like element ISRm2011-2 family transposase	-
JFX10_RS04495	1016962..1018221	-	1260	WP_010975503.1	group II intron reverse transcriptase/maturase	-
JFX10_RS04500	1018769..1019222	-	454	Protein_896	IS630-like element ISRm2011-2 family transposase	-
JFX10_RS04505	1019382..1019936	-	555	WP_198868371.1	RES family NAD+ phosphorylase	Toxin
JFX10_RS04510	1019943..1020314	-	372	WP_003534594.1	DUF2384 domain-containing protein	Antitoxin
JFX10_RS04515	1020391..1021176	-	786	WP_198868372.1	crotonase/enoyl-CoA hydratase family protein	-
JFX10_RS04520	1021173..1022042	-	870	WP_198868373.1	hydroxymethylglutaryl-CoA lyase	-
JFX10_RS04525	1022039..1024027	-	1989	WP_015243150.1	acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha	-
JFX10_RS04530	1024044..1024427	-	384	WP_010975573.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..1719217	1719217

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (23-177)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (71-120)

Sequences

Toxin

Protein

• Length: 185 a.a.
• Molecular weight: 20416.32 Da
• Isoelectric Point: 6.2239

>T184315 WP_198868371.1 NZ_CP066358:c1019936-1019382 [Sinorhizobium meliloti]
MRKHIPPASPPDPEPTSPVELITLWRAFVPRWAHMPLSGAGAARFGGRWNPIGMPAIYAARELSTAWAEYNQGFVQHPAL
IVQLELRGARLADLTDASVLLELGVDEAIHRCEWRDALDKGAVPETHRLQSELLARDYHGVIYPSFMSPGGTCVALWRWN
GAEEPRLDVIDPDGRLPKSPASWV

Nucleotide

• Length: 555 bp

>T184315 NZ_CP087611:c4683591-4683214 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTGCTGGACCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACTCGCGA
CAATTACAGAACGGTAAATAACATTACCCTAGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13113.26 Da
• Isoelectric Point: 9.9996

>AT184315 WP_003534594.1 NZ_CP066358:c1020314-1019943 [Sinorhizobium meliloti]
MMAVDFQIPAARFGDDHSPFLSARLVADRLGVTLAELAKLIGVARNTLTAKSGARKVDSALSKVVRILAMASEMAGDEAR
AVIWFKHQPIPGWAGKTAFDLVGEGKADKVLAYLESVRAGVYA

Nucleotide

• Length: 372 bp

>AT184315 NZ_CP087611:c4684049-4683681 [Klebsiella pneumoniae subsp. pneumoniae]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCATGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: H0G7T7