Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RelE-RelB |
| Location | 186602..187203 | Replicon | plasmid pUSDA76 |
| Accession | NZ_CP066357 | ||
| Organism | Bradyrhizobium elkanii USDA 76 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | A0A4Y3ZSU1 |
| Locus tag | JEY66_RS43945 | Protein ID | WP_018273807.1 |
| Coordinates | 186907..187203 (+) | Length | 99 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | A0A4Y3ZS41 |
| Locus tag | JEY66_RS43940 | Protein ID | WP_018273808.1 |
| Coordinates | 186602..186910 (+) | Length | 103 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JEY66_RS43915 (JEY66_43720) | 182459..182881 | + | 423 | Protein_169 | hypothetical protein | - |
| JEY66_RS43920 (JEY66_43725) | 182878..183291 | + | 414 | WP_018273814.1 | hypothetical protein | - |
| JEY66_RS43925 (JEY66_43730) | 183689..184276 | + | 588 | WP_026193407.1 | hypothetical protein | - |
| JEY66_RS43930 (JEY66_43735) | 184571..185497 | + | 927 | WP_018273812.1 | zincin-like metallopeptidase domain-containing protein | - |
| JEY66_RS43935 (JEY66_43740) | 186157..186468 | + | 312 | WP_018273809.1 | DUF736 domain-containing protein | - |
| JEY66_RS43940 (JEY66_43745) | 186602..186910 | + | 309 | WP_018273808.1 | type II toxin-antitoxin system RelB/DinJ family antitoxin | Antitoxin |
| JEY66_RS43945 (JEY66_43750) | 186907..187203 | + | 297 | WP_018273807.1 | type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family | Toxin |
| JEY66_RS43950 (JEY66_43755) | 187493..188674 | - | 1182 | WP_026193406.1 | RHE_PE00001 family protein | - |
| JEY66_RS43955 (JEY66_43760) | 188980..189375 | - | 396 | WP_018273805.1 | type II toxin-antitoxin system VapC family toxin | - |
| JEY66_RS43960 (JEY66_43765) | 189375..189620 | - | 246 | WP_018273804.1 | type II toxin-antitoxin system VapB family antitoxin | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..402731 | 402731 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 99 a.a. Molecular weight: 11023.40 Da Isoelectric Point: 5.0992
>T184314 WP_018273807.1 NZ_CP066357:186907-187203 [Bradyrhizobium elkanii USDA 76]
VKLVWSRFALSDRDSIFSYIEAENPRAAVHVDEQIANAARRLLDFPDSGRPGRVAGTRELVIPRTSYVAAYLVDGDTVRI
LRVLHGAQMWPDELTNDD
VKLVWSRFALSDRDSIFSYIEAENPRAAVHVDEQIANAARRLLDFPDSGRPGRVAGTRELVIPRTSYVAAYLVDGDTVRI
LRVLHGAQMWPDELTNDD
Download Length: 297 bp
>T184314 NZ_CP087611:c4644373-4643840 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA
Antitoxin
Download Length: 103 a.a. Molecular weight: 11396.84 Da Isoelectric Point: 4.8950
>AT184314 WP_018273808.1 NZ_CP066357:186602-186910 [Bradyrhizobium elkanii USDA 76]
MASNALVQTRIDADVKEKATAVLENMGLTVSDAVRILLTRTANEGMLPLELVSNSQAYDTWFREKVRQALADTRPDIDDS
EVEAHFAQRRAAALRKAPEQER
MASNALVQTRIDADVKEKATAVLENMGLTVSDAVRILLTRTANEGMLPLELVSNSQAYDTWFREKVRQALADTRPDIDDS
EVEAHFAQRRAAALRKAPEQER
Download Length: 309 bp
>AT184314 NZ_CP087611:c4644650-4644384 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4Y3ZSU1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4Y3ZS41 |