Detailed information of TA system TA184312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 8255789..8257401
• Replicon: chromosome
• Accession: NZ_CP066355
• Organism: Bradyrhizobium diazoefficiens strain USDA 122-Bd

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: A0A837C678
• Locus tag: JEY62_RS38635
• Protein ID: WP_049832575.1
• Coordinates: 8255789..8256712 (-)
• Length: 308 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: JEY62_RS38640
• Protein ID: WP_231166619.1
• Coordinates: 8256709..8257401 (-)
• Length: 231 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JEY62_RS38605 (JEY62_38355)	8250857..8252026	+	1170	WP_039187218.1	tyrosine-type recombinase/integrase	-
JEY62_RS38610 (JEY62_38360)	8252061..8252579	+	519	WP_131234233.1	hypothetical protein	-
JEY62_RS38615 (JEY62_38365)	8252595..8253308	+	714	WP_131234232.1	hypothetical protein	-
JEY62_RS38620 (JEY62_38370)	8253385..8253591	+	207	WP_028175471.1	helix-turn-helix domain-containing protein	-
JEY62_RS38625 (JEY62_38375)	8253623..8254549	+	927	WP_157862142.1	toprim domain-containing protein	-
JEY62_RS38630 (JEY62_38380)	8254552..8255634	+	1083	WP_049832576.1	AAA family ATPase	-
JEY62_RS38635 (JEY62_38385)	8255789..8256712	-	924	WP_049832575.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
JEY62_RS38640 (JEY62_38390)	8256709..8257401	-	693	WP_231166619.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
JEY62_RS38645 (JEY62_38395)	8257575..8261633	+	4059	WP_028175467.1	helix-turn-helix transcriptional regulator	-
JEY62_RS38650 (JEY62_38400)	8261760..8262317	+	558	WP_074077613.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	8252061..8273195	21134

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (39-276)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (37-81)

Sequences

Toxin

Protein

• Length: 308 a.a.
• Molecular weight: 34339.39 Da
• Isoelectric Point: 4.9777

>T184312 WP_049832575.1 NZ_CP066355:c8256712-8255789 [Bradyrhizobium diazoefficiens]
MIGKPLKDPGASIRQRLLNHAKEHGDEYQRILTRYAIERLLFRLSQTEARERYVLKGAMLFVTWPEQVFRPTGDLDLLGQ
GDPDPAAIIDLFTLICQVEAPADGIIFDPSTLQVEAVREEEKYQGVRLSLRGRLGTAVIPVQVDIGFGDSVHPAPSRNNF
PSLLPDLPAADVLMYPPETVIAEKFEAMIRFGEANGRIKDFHDIWVTARTFAFNLSTLVEAVSGTLQRRETAFPTEMPVA
LTSQFAEMADKQALWTGFLRRNPPTLPPPPLGDLLAELRRFFEPVLAALPLPESATGRWNPDRGAWE

Nucleotide

• Length: 924 bp

>T184312 NZ_CP087611:c3885691-3885374 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 231 a.a.
• Molecular weight: 25491.54 Da
• Isoelectric Point: 10.3648

>AT184312 WP_231166619.1 NZ_CP066355:c8257401-8256709 [Bradyrhizobium diazoefficiens]
MTSAANLQMLFNVDKSADVPTKDNKMTQRHVLPYRERALNLVRARGIARARDFKAAGIPLVYLKRLTDTGELVRLGRGLY
QDPDRVGEDVAHNLAEAARAVPNGVVSLASALRYHGLTTQLPHAVWLTIPHKARTPKAIGFPLEIVRATGDVMTAGVEHV
QVEGVDVPIYGVAKTIADCFKHRRHIGEDVAIEALRDALRLRKTTVSDVMRYAEIDRVAERIRPVLKALQ

Nucleotide

• Length: 693 bp

>AT184312 NZ_CP087611:c3885381-3885094 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A837C678