Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rlegTA/AbiEii-AbiEi
Location 1463601..1465131 Replicon chromosome
Accession NZ_CP066355
Organism Bradyrhizobium diazoefficiens strain USDA 122-Bd

Toxin (Protein)


Gene name rlegT Uniprot ID A0A562QN92
Locus tag JEY62_RS06650 Protein ID WP_018647871.1
Coordinates 1463601..1464521 (-) Length 307 a.a.

Antitoxin (Protein)


Gene name rlegA Uniprot ID A0A810CIT3
Locus tag JEY62_RS06655 Protein ID WP_026312796.1
Coordinates 1464511..1465131 (-) Length 207 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JEY62_RS06635 (JEY62_06585) 1459573..1460094 - 522 WP_011084725.1 exonuclease subunit SbcD -
JEY62_RS06640 (JEY62_06590) 1460103..1462211 - 2109 WP_011084726.1 DUF87 domain-containing protein -
JEY62_RS06645 (JEY62_06595) 1462219..1463523 - 1305 WP_028176022.1 SIR2 family protein -
JEY62_RS06650 (JEY62_06600) 1463601..1464521 - 921 WP_018647871.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
JEY62_RS06655 (JEY62_06605) 1464511..1465131 - 621 WP_026312796.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein Antitoxin
JEY62_RS06660 (JEY62_06610) 1465345..1466091 - 747 WP_018647872.1 DCL family protein -
JEY62_RS06665 (JEY62_06615) 1466066..1468546 - 2481 WP_231166584.1 AAA family ATPase -
JEY62_RS06670 (JEY62_06620) 1468539..1468766 - 228 WP_131234255.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(38-264)

Antitoxin

Transcriptional regulator, AbiEi antitoxin

(12-56)


Sequences

Toxin        


Download         Length: 307 a.a.        Molecular weight: 34027.14 Da        Isoelectric Point: 5.2727

>T184310 WP_018647871.1 NZ_CP066355:c1464521-1463601 [Bradyrhizobium diazoefficiens]
MAREPRNIGASVRARLLDRARAERSDFQILLTRYALERLLYRLSASPHRDRFILKGAMLFVTWIADPFRPTRDLDLLGHG
DRDTEAIAETFRAICAQPVVDDGVTFDVAALMATPIREELEYGGVRVRTTATIAGARIPIQVDIGFGDAITPAAVEIDYP
TLLDAPTPHLRAYPVETVVAEKFAALVTLGVANSRLKDFYDLWVISRTFELRRAALAEAIQRTFERRGTVLPSVVPVGLT
DEFAETWAAQWRAFLERDRMAAAPDAFAVTVADLRLFLMPLVVGLKEERIWPPSGPWSPGAAIDEA

Download         Length: 921 bp

>T184310 NZ_CP087611:1974305-1974407 [Klebsiella pneumoniae subsp. pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 207 a.a.        Molecular weight: 23385.28 Da        Isoelectric Point: 10.0691

>AT184310 WP_026312796.1 NZ_CP066355:c1465131-1464511 [Bradyrhizobium diazoefficiens]
MPSTSAQRLRALDLLKTRGMLRLKDFIAEGIGPETLARLVREEAVVRPARGLYQLPDAHLEAAHTLAEAAVLVPKGVVCL
TSALQYHELTLQMPSAVWMAIDRSAWRPKIDYPPIRFVRFTGSALTEGIERHRIENVDVPITDPARTIVDCFRYRTKIGL
DVAMEGLREGLRRRRCKPDQLWHYARKARVWSVMRPYVEAMVSDGA

Download         Length: 621 bp

>AT184310 NZ_CP087611:c1974414-1974269 [Klebsiella pneumoniae subsp. pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A562QN92

Antitoxin

Source ID Structure
AlphaFold DB A0A810CIT3

References