Detailed information of TA system TA184309

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 309422..310018
• Replicon: plasmid p1S06B
• Accession: NZ_CP066352
• Organism: Bradyrhizobium japonicum strain S06B-BJ

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: JEY30_RS48370
• Protein ID: WP_248890119.1
• Coordinates: 309422..309718 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: JEY30_RS48375
• Protein ID: WP_248889774.1
• Coordinates: 309722..310018 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JEY30_RS48345 (JEY30_47940)	305408..306672	+	1265	Protein_302	IS256 family transposase	-
JEY30_RS48350 (JEY30_47945)	306718..307803	+	1086	WP_283258621.1	acyltransferase	-
JEY30_RS48355 (JEY30_47950)	307942..308112	+	171	WP_248889772.1	hypothetical protein	-
JEY30_RS48360 (JEY30_47955)	308193..308537	+	345	WP_248889773.1	hypothetical protein	-
JEY30_RS48365 (JEY30_47960)	308738..309298	+	561	Protein_306	DNA ligase	-
JEY30_RS48370 (JEY30_47965)	309422..309718	+	297	WP_248890119.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JEY30_RS48375 (JEY30_47970)	309722..310018	+	297	WP_248889774.1	putative addiction module antidote protein	Antitoxin
JEY30_RS48380 (JEY30_47975)	310157..310717	+	561	WP_248889775.1	hypothetical protein	-
JEY30_RS48385 (JEY30_47980)	310854..311150	-	297	WP_248889777.1	hypothetical protein	-
JEY30_RS48390 (JEY30_47985)	311366..312328	+	963	WP_248889778.1	transposase family protein	-
JEY30_RS51970	312413..312835	+	423	WP_018273743.1	transposase	-
JEY30_RS48400 (JEY30_47995)	312850..313188	+	339	WP_248888238.1	IS66 family insertion sequence element accessory protein TnpB	-
JEY30_RS48405 (JEY30_48000)	313239..314789	+	1551	WP_035681142.1	IS66 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..572704	572704
	-	flank	IS/Tn	-	-	305668..306672	1004

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (4-90)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10920.42 Da
• Isoelectric Point: 5.9023

>T184309 WP_248890119.1 NZ_CP066352:309422-309718 [Bradyrhizobium japonicum]
MPAIRETEQFSSWLSDLRDVRAKAKVLVRVERLAKGNAGDVKPVGEGISELRIHYGAGYRVYFTQRGEELILLLCGGDKD
SQDKDIANAKKIASELEE

Nucleotide

• Length: 297 bp

>T184309 NZ_CP087611:857500-857910 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAAATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10529.93 Da
• Isoelectric Point: 5.6227

>AT184309 WP_248889774.1 NZ_CP066352:309722-310018 [Bradyrhizobium japonicum]
MARTRPFDAAEYLDSPEAIAAYLSEAFETNDPAFITDAIGTVARARGMTAVAKDAGLSREHLYKALSSEGRPEFGTVLKV
LESLGIHLHAEPRVAKVA

Nucleotide

• Length: 297 bp

>AT184309 NZ_CP087611:857253-857519 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA