Detailed information of TA system TA184308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-Phd
• Location: 3284553..3285256
• Replicon: chromosome
• Accession: NZ_CP066351
• Organism: Bradyrhizobium japonicum strain S06B-BJ

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JEY30_RS15630
• Protein ID: WP_038956523.1
• Coordinates: 3284553..3284978 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A845J034
• Locus tag: JEY30_RS15635
• Protein ID: WP_014497445.1
• Coordinates: 3284975..3285256 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JEY30_RS15600 (JEY30_15435)	3280442..3280852	+	411	WP_038936928.1	acyl-CoA thioesterase	-
JEY30_RS15605 (JEY30_15440)	3281073..3281264	+	192	WP_014497451.1	hypothetical protein	-
JEY30_RS15610 (JEY30_15445)	3281499..3281891	-	393	WP_014497450.1	nuclear transport factor 2 family protein	-
JEY30_RS15615 (JEY30_15450)	3282294..3282758	-	465	WP_038956521.1	nuclear transport factor 2 family protein	-
JEY30_RS15620 (JEY30_15455)	3282913..3283611	+	699	WP_038937242.1	HAD-IA family hydrolase	-
JEY30_RS15625 (JEY30_15460)	3283703..3284548	+	846	WP_038956522.1	DeoR/GlpR family DNA-binding transcription regulator	-
JEY30_RS15630 (JEY30_15465)	3284553..3284978	-	426	WP_038956523.1	type II toxin-antitoxin system VapC family toxin	Toxin
JEY30_RS15635 (JEY30_15470)	3284975..3285256	-	282	WP_014497445.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JEY30_RS15640 (JEY30_15475)	3285487..3287037	+	1551	WP_014497444.1	glycerol-3-phosphate dehydrogenase	-
JEY30_RS15645 (JEY30_15480)	3287034..3288170	+	1137	WP_038956524.1	ABC transporter ATP-binding protein	-
JEY30_RS15650 (JEY30_15485)	3288183..3289268	+	1086	WP_038956525.1	ABC transporter ATP-binding protein	-
JEY30_RS15655 (JEY30_15490)	3289268..3290242	+	975	WP_028160307.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (14-77)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15245.51 Da
• Isoelectric Point: 7.2061

>T184308 WP_038956523.1 NZ_CP066351:c3284978-3284553 [Bradyrhizobium japonicum]
VKLLLDTNVLSEVQRAAPSPKVLAWLDTVDEDRAFISAASIAELRRGIALLDDGRRRAALAAWLAHDLPARFAQRILPID
HAVAERWGDLMAQSRRTGVALSAMDGFLAATAFANNLTLVTRNVTDFAAFGVPLLNPWSGA

Nucleotide

• Length: 426 bp

>T184308 NZ_CP087611:710953-711294 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAAACTCTACCTGCTATAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAGTCTGGCAAATGCTACTTAC
ACGCCTGCTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCTGTGATGAAACCGTTATACAGGAACATATCG
ATGCCGGTATTACGCTGGCTGATGCCGTTAATTTTCTGGTGGAAAAATACGGACTGGTTCGTATCGACAGGAATGGATTT
GTCTGGCAGGAACAATCACCTTATCTTCGGGCCGTTGATATTCTGCGAGCGCGGCAGGCAACTGGCTTATTGCGACAAAG
CCGTAACAACGCAATACGTTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10236.45 Da
• Isoelectric Point: 5.1409

>AT184308 WP_014497445.1 NZ_CP066351:c3285256-3284975 [Bradyrhizobium japonicum]
MTDRSSAPDSPPTDTWTLANAKARLSEVVDRAQTGPQIITRHGRPNAVVVSADEWARKTARKGTLAEFLLASPLRGADLE
LERVQDEPRDEMP

Nucleotide

• Length: 282 bp

>AT184308 NZ_CP087611:710615-710932 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGCAACATTACATGGGGCCTGCAGCGGGATACCACGCCGCGTCTGGGAGCCCGTCTGGTACAGGAGGGGAACCAACT
GCATTATCTGGCTGACCGGGCCAGTATGACCGGTAAATTCAGTGACGCCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGATCACTGGTGAAATGAATCCTCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGCCGTTTACCCCATCCAACGATAA

Structures

Antitoxin

• AlphaFold DB: A0A845J034