Detailed information of TA system TA184307

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3267830..3268401
• Replicon: chromosome
• Accession: NZ_CP066351
• Organism: Bradyrhizobium japonicum strain S06B-BJ

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A845IZB5
• Locus tag: JEY30_RS15555
• Protein ID: WP_014497460.1
• Coordinates: 3267830..3268183 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: JEY30_RS15560
• Protein ID: WP_080583987.1
• Coordinates: 3268180..3268401 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JEY30_RS15530 (JEY30_15365)	3264047..3264283	+	237	WP_014497465.1	hypothetical protein	-
JEY30_RS15535 (JEY30_15370)	3264382..3264627	+	246	WP_038956514.1	hypothetical protein	-
JEY30_RS15540 (JEY30_15375)	3264742..3265905	+	1164	WP_038956515.1	crosslink repair DNA glycosylase YcaQ family protein	-
JEY30_RS15545 (JEY30_15380)	3265924..3266352	-	429	WP_038956516.1	DUF2809 domain-containing protein	-
JEY30_RS15550 (JEY30_15385)	3266433..3267764	+	1332	WP_038936921.1	MFS transporter	-
JEY30_RS15555 (JEY30_15390)	3267830..3268183	-	354	WP_014497460.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JEY30_RS15560 (JEY30_15395)	3268180..3268401	-	222	WP_080583987.1	antitoxin MazE family protein	Antitoxin
JEY30_RS15565 (JEY30_15400)	3268580..3269686	+	1107	WP_028170414.1	efflux RND transporter periplasmic adaptor subunit	-
JEY30_RS15570 (JEY30_15405)	3269920..3273057	+	3138	WP_014497458.1	efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-104)

Antitoxin

• Antitoxin MazE-like (12-73)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12687.49 Da
• Isoelectric Point: 6.3275

>T184307 WP_014497460.1 NZ_CP066351:c3268183-3267830 [Bradyrhizobium japonicum]
MKRGEIWTVAGGKDYAGKPRPVVIVQDDSFDATNSITVCAFTTDETEAPLFRLKVEPNDNNGLRVPSRLMVDKITTVPKA
KVGTLVGRLDDDDLLRLNRAVLVFLGLAGSQRSASAD

Nucleotide

• Length: 354 bp

>T184307 NZ_CP087611:325782-326150 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8248.25 Da
• Isoelectric Point: 10.4527

>AT184307 WP_080583987.1 NZ_CP066351:c3268401-3268180 [Bradyrhizobium japonicum]
MSPSKPKPSRLKVQAHRDRLRAQGLRPIQIWVPDIRSPSFRSEAHRQSLAVATSVHASEDQGFIDAVSDWTDE

Nucleotide

• Length: 222 bp

>AT184307 NZ_CP087611:325564-325785 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A845IZB5