Detailed information of TA system TA184306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 6733..7322
• Replicon: plasmid pLMG696-1
• Accession: NZ_CP066344
• Organism: Xanthomonas citri pv. durantae strain LMG696

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: M4U123
• Locus tag: Xdur_RS23255
• Protein ID: WP_015463824.1
• Coordinates: 6733..7065 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: M4UHU6
• Locus tag: Xdur_RS23260
• Protein ID: WP_011052949.1
• Coordinates: 7065..7322 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Xdur_RS23235 (Xdur_023160)	5213..5434	-	222	WP_011052944.1	hypothetical protein	-
Xdur_RS23240 (Xdur_023165)	5724..6260	-	537	WP_015463823.1	plasmid pRiA4b ORF-3 family protein	-
Xdur_RS23245 (Xdur_023170)	6250..6462	-	213	WP_011052947.1	hypothetical protein	-
Xdur_RS23250 (Xdur_023175)	6459..6710	-	252	WP_125459571.1	hypothetical protein	-
Xdur_RS23255 (Xdur_023180)	6733..7065	-	333	WP_015463824.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
Xdur_RS23260 (Xdur_023185)	7065..7322	-	258	WP_011052949.1	antitoxin	Antitoxin
Xdur_RS23265 (Xdur_023190)	7528..8130	+	603	WP_011052950.1	recombinase family protein	-
Xdur_RS23270 (Xdur_023195)	8114..8418	+	305	Protein_9	DUF4158 domain-containing protein	-
Xdur_RS23275 (Xdur_023200)	8585..11545	-	2961	WP_015472939.1	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..66975	66975

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11707.46 Da
• Isoelectric Point: 9.1975

>T184306 WP_015463824.1 NZ_CP066344:c7065-6733 [Xanthomonas citri pv. durantae]
MDRGDVYLVSLDPTSGHEQQGTRPVLIVSPSAFNRLTKTPVVLPITSGGNFARTAGFTVSLMGAGTNTTGVVRCDQPRAL
DLASRRARKLETVPSPIMDEVLAKLSAILE

Nucleotide

• Length: 333 bp

>T184306 NZ_CP087610:4824-5090 [Klebsiella pneumoniae subsp. pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.70 Da
• Isoelectric Point: 4.7164

>AT184306 WP_011052949.1 NZ_CP066344:c7322-7065 [Xanthomonas citri pv. durantae]
MHTTNLRKVGGSIMLAVPPAILDMLRLRAGATVGLAVDHGRLVIEPTLRPYYSLDELLAQCDASAELSTEDREWLDAKPV
GSELL

Nucleotide

• Length: 258 bp

>AT184306 NZ_CP087610:4616-4840 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: M4U123

Antitoxin

• AlphaFold DB: M4UHU6