Detailed information of TA system TA184299

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3408683..3409600
• Replicon: chromosome
• Accession: NZ_CP066337
• Organism: Bacillus velezensis strain Mr12

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: JFU10_RS17110
• Protein ID: WP_057079950.1
• Coordinates: 3408854..3409600 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: JFU10_RS17105
• Protein ID: WP_003154807.1
• Coordinates: 3408683..3408853 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFU10_RS17070	3403916..3405538	+	1623	WP_198863658.1	pyocin knob domain-containing protein	-
JFU10_RS17075	3405551..3405922	+	372	WP_070081681.1	hypothetical protein	-
JFU10_RS17080	3405927..3406124	+	198	WP_071391956.1	XkdX family protein	-
JFU10_RS17085	3406181..3406942	+	762	WP_071391955.1	phage portal protein	-
JFU10_RS17090	3406994..3407257	+	264	WP_003154815.1	hemolysin XhlA family protein	-
JFU10_RS17095	3407271..3407534	+	264	WP_003154813.1	phage holin	-
JFU10_RS17100	3407548..3408426	+	879	WP_045207177.1	N-acetylmuramoyl-L-alanine amidase	-
JFU10_RS17105	3408683..3408853	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
JFU10_RS17110	3408854..3409600	-	747	WP_057079950.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
JFU10_RS17115	3409705..3410703	-	999	WP_003154805.1	inorganic phosphate transporter	-
JFU10_RS17120	3410716..3411333	-	618	WP_003154804.1	DUF47 domain-containing protein	-
JFU10_RS17125	3411619..3412935	-	1317	WP_003154801.1	amino acid permease	-
JFU10_RS17130	3413258..3414208	+	951	WP_039251754.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29033.52 Da
• Isoelectric Point: 4.7755

>T184299 WP_057079950.1 NZ_CP066337:c3409600-3408854 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T184299 NZ_CP087606:c4891275-4890991 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT184299 WP_003154807.1 NZ_CP066337:c3408853-3408683 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT184299 NZ_CP087606:c4891507-4891265 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14