Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2654555..2655192 Replicon chromosome
Accession NZ_CP066337
Organism Bacillus velezensis strain Mr12

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag JFU10_RS12950 Protein ID WP_003156187.1
Coordinates 2654842..2655192 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag JFU10_RS12945 Protein ID WP_003156188.1
Coordinates 2654555..2654836 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFU10_RS12925 2650920..2651519 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
JFU10_RS12930 2651612..2651977 + 366 WP_070081400.1 holo-ACP synthase -
JFU10_RS12935 2652142..2653149 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
JFU10_RS12940 2653266..2654435 + 1170 WP_198863500.1 alanine racemase -
JFU10_RS12945 2654555..2654836 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
JFU10_RS12950 2654842..2655192 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
JFU10_RS12955 2655310..2656131 + 822 WP_003156182.1 STAS domain-containing protein -
JFU10_RS12960 2656136..2656501 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
JFU10_RS12965 2656504..2656905 + 402 WP_003156178.1 anti-sigma regulatory factor -
JFU10_RS12970 2656917..2657924 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
JFU10_RS12975 2657988..2658317 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
JFU10_RS12980 2658314..2658796 + 483 WP_003156173.1 anti-sigma B factor RsbW -
JFU10_RS12985 2658762..2659550 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
JFU10_RS12990 2659550..2660152 + 603 WP_003156170.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T184298 WP_003156187.1 NZ_CP066337:2654842-2655192 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T184298 NZ_CP087606:c4801169-4800636 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT184298 WP_003156188.1 NZ_CP066337:2654555-2654836 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT184298 NZ_CP087606:c4801446-4801180 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References