Detailed information of TA system TA184294

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 71163..72190
• Replicon: plasmid unnamed2
• Accession: NZ_CP066312
• Organism: Azotobacter chroococcum strain HR1

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: GKQ51_RS23710
• Protein ID: WP_090624017.1
• Coordinates: 71163..71735 (-)
• Length: 191 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: GKQ51_RS23715
• Protein ID: WP_090624018.1
• Coordinates: 71732..72190 (-)
• Length: 153 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKQ51_RS23675	66857..67213	-	357	WP_198868240.1	hypothetical protein	-
GKQ51_RS23680	67453..67788	+	336	WP_198868241.1	hypothetical protein	-
GKQ51_RS23685	68020..68259	+	240	WP_090624039.1	hypothetical protein	-
GKQ51_RS23690	68311..68607	+	297	WP_198868242.1	HU family DNA-binding protein	-
GKQ51_RS23695	68959..70188	+	1230	WP_198868243.1	ATP-binding protein	-
GKQ51_RS23700	70376..70603	+	228	WP_090624014.1	CopG family transcriptional regulator	-
GKQ51_RS23705	70587..70853	+	267	WP_198868244.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GKQ51_RS23710	71163..71735	-	573	WP_090624017.1	PIN domain-containing protein	Toxin
GKQ51_RS23715	71732..72190	-	459	WP_090624018.1	helix-turn-helix domain-containing protein	Antitoxin
GKQ51_RS23720	72387..72672	+	286	Protein_73	type II toxin-antitoxin system MqsR family toxin	-
GKQ51_RS23725	72675..73076	+	402	WP_090624020.1	type II toxin-antitoxin system MqsA family antitoxin	-
GKQ51_RS23730	73137..73316	-	180	Protein_75	site-specific integrase	-
GKQ51_RS23735	73323..73555	-	233	Protein_76	helix-turn-helix domain-containing protein	-
GKQ51_RS23740	73679..74275	-	597	WP_198868268.1	putative adenosine monophosphate-protein transferase Fic	-
GKQ51_RS23745	74278..74445	-	168	WP_089170224.1	YhfG family protein	-
GKQ51_RS23750	74642..75598	-	957	WP_198868245.1	zinc-binding alcohol dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..76351	76351

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-114)

Antitoxin

• SinI-like, DNA-binding domain (81-126)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 21564.82 Da
• Isoelectric Point: 6.2271

>T184294 WP_090624017.1 NZ_CP066312:c71735-71163 [Azotobacter chroococcum]
VSSNYTVVYDACVMYPAPLRSLLMYLALSGQFRARWSETIHQEWMRNLLADRPDLSPDTVGRVRELMDRHVPGALVTGFE
SLIPGITLPDPEDRHVVAAAVQTRAEAIVTFNLKDFPDQDLSPFGVRAIHPDDFITDLMELHIGAVLEAVRRHRASLKNP
PFSSREYLDCLLKQRLPETVSRLRAFELLI

Nucleotide

• Length: 573 bp

>T184294 NZ_CP087606:878632-879039 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 153 a.a.
• Molecular weight: 16670.06 Da
• Isoelectric Point: 4.8545

>AT184294 WP_090624018.1 NZ_CP066312:c72190-71732 [Azotobacter chroococcum]
MNRLQETLPSAEEATLAKLCSQELSAIIETRAETQTLSISDPKGGIRSVNIPVSALRLLVDVLTELGEGNSVRLVPVHAE
LTTQEAADLLNVSRPTLIKLLDEGAIPYHRAGNRRKVKFADLRLYQERLEQERLAALAELSALDQALGLGYE

Nucleotide

• Length: 459 bp

>AT184294 NZ_CP087606:878385-878651 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA