Detailed information of TA system TA184293

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 70376..70853
• Replicon: plasmid unnamed2
• Accession: NZ_CP066312
• Organism: Azotobacter chroococcum strain HR1

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: GKQ51_RS23705
• Protein ID: WP_198868244.1
• Coordinates: 70587..70853 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: GKQ51_RS23700
• Protein ID: WP_090624014.1
• Coordinates: 70376..70603 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKQ51_RS23675	66857..67213	-	357	WP_198868240.1	hypothetical protein	-
GKQ51_RS23680	67453..67788	+	336	WP_198868241.1	hypothetical protein	-
GKQ51_RS23685	68020..68259	+	240	WP_090624039.1	hypothetical protein	-
GKQ51_RS23690	68311..68607	+	297	WP_198868242.1	HU family DNA-binding protein	-
GKQ51_RS23695	68959..70188	+	1230	WP_198868243.1	ATP-binding protein	-
GKQ51_RS23700	70376..70603	+	228	WP_090624014.1	CopG family transcriptional regulator	Antitoxin
GKQ51_RS23705	70587..70853	+	267	WP_198868244.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GKQ51_RS23710	71163..71735	-	573	WP_090624017.1	PIN domain-containing protein	-
GKQ51_RS23715	71732..72190	-	459	WP_090624018.1	helix-turn-helix domain-containing protein	-
GKQ51_RS23720	72387..72672	+	286	Protein_73	type II toxin-antitoxin system MqsR family toxin	-
GKQ51_RS23725	72675..73076	+	402	WP_090624020.1	type II toxin-antitoxin system MqsA family antitoxin	-
GKQ51_RS23730	73137..73316	-	180	Protein_75	site-specific integrase	-
GKQ51_RS23735	73323..73555	-	233	Protein_76	helix-turn-helix domain-containing protein	-
GKQ51_RS23740	73679..74275	-	597	WP_198868268.1	putative adenosine monophosphate-protein transferase Fic	-
GKQ51_RS23745	74278..74445	-	168	WP_089170224.1	YhfG family protein	-
GKQ51_RS23750	74642..75598	-	957	WP_198868245.1	zinc-binding alcohol dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..76351	76351

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10262.86 Da
• Isoelectric Point: 10.1537

>T184293 WP_198868244.1 NZ_CP066312:70587-70853 [Azotobacter chroococcum]
MAWTIDYTQTALNQLRKLDKPSARRILDFMEERVALREDPRSTGRALTGPLGGLWRYRVGDFRVICEIQDGALRVLVLEL
GNRREVYR

Nucleotide

• Length: 267 bp

>T184293 NZ_CP087606:743661-744146 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8695.78 Da
• Isoelectric Point: 4.3775

>AT184293 WP_090624014.1 NZ_CP066312:70376-70603 [Azotobacter chroococcum]
MPTSIRLAPEIEQRLDFLAANTGRTKAYYLREIIERGLDEMEDYYLAAEVLERVRKGQEQVYSSADVRKDLGLDD

Nucleotide

• Length: 228 bp

>AT184293 NZ_CP087606:743371-743664 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA