Detailed information of TA system TA184292

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/RHH(antitoxin)
• Location: 7269..7822
• Replicon: plasmid unnamed2
• Accession: NZ_CP066312
• Organism: Azotobacter chroococcum strain HR1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: GKQ51_RS23400
• Protein ID: WP_136887983.1
• Coordinates: 7269..7562 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GKQ51_RS23405
• Protein ID: WP_136887984.1
• Coordinates: 7550..7822 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKQ51_RS23380	4658..5011	-	354	WP_198868248.1	hypothetical protein	-
GKQ51_RS23385	6079..6369	+	291	WP_136887981.1	nucleotidyltransferase family protein	-
GKQ51_RS23390	6475..6756	-	282	Protein_7	PIN domain nuclease	-
GKQ51_RS23395	6865..7140	+	276	WP_136887982.1	hypothetical protein	-
GKQ51_RS23400	7269..7562	-	294	WP_136887983.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GKQ51_RS23405	7550..7822	-	273	WP_136887984.1	CopG family ribbon-helix-helix protein	Antitoxin
GKQ51_RS23410	8876..9028	-	153	Protein_11	transcriptional regulator	-
GKQ51_RS23415	9441..9591	-	151	Protein_12	integrase	-
GKQ51_RS23420	10097..10237	-	141	WP_198868249.1	hypothetical protein	-
GKQ51_RS23425	10287..10370	+	84	Protein_14	transposase	-
GKQ51_RS23430	10375..10542	+	168	Protein_15	type II toxin-antitoxin system RelE/ParE family toxin	-
GKQ51_RS23435	10564..10791	+	228	WP_198868250.1	MarR family transcriptional regulator	-
GKQ51_RS23440	10724..11413	-	690	Protein_17	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..76351	76351

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-95)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10833.33 Da
• Isoelectric Point: 5.1372

>T184292 WP_136887983.1 NZ_CP066312:c7562-7269 [Azotobacter chroococcum]
VPRVVVTEGAAQGLERCRLFLAEKSPQVASRAGQAIEHQFALLEKSPDIGRPFPELPELRELIIAFGDSGYVALYRYEPA
DDTVYVLAFRHQKEAGY

Nucleotide

• Length: 294 bp

>T184292 NZ_CP087606:356143-356490 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10627.83 Da
• Isoelectric Point: 5.6224

>AT184292 WP_136887984.1 NZ_CP066312:c7822-7550 [Azotobacter chroococcum]
MATSIKIDDELKARIQHLACQRQRSAHWIMREAIAQYVEREEARESFKQEALASWAAYRETGRHLTGQETREWLNTWGKE
EETAVPECHE

Nucleotide

• Length: 273 bp

>AT184292 NZ_CP087606:356490-356789 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA