Detailed information of TA system TA184291

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 224100..224743
• Replicon: plasmid unnamed1
• Accession: NZ_CP066311
• Organism: Azotobacter chroococcum strain HR1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: GKQ51_RS22940
• Protein ID: WP_198868152.1
• Coordinates: 224100..224498 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A0C4WT59
• Locus tag: GKQ51_RS22945
• Protein ID: WP_040107521.1
• Coordinates: 224513..224743 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKQ51_RS22940	224100..224498	-	399	WP_198868152.1	type II toxin-antitoxin system VapC family toxin	Toxin
GKQ51_RS22945	224513..224743	-	231	WP_040107521.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GKQ51_RS22950	224921..226681	-	1761	WP_198868153.1	DUF4942 domain-containing protein	-
GKQ51_RS22955	226804..227406	+	603	WP_198868154.1	class I SAM-dependent methyltransferase	-
GKQ51_RS22960	227651..228844	+	1194	WP_039802387.1	IS4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..307780	307780

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14478.65 Da
• Isoelectric Point: 6.7037

>T184291 WP_198868152.1 NZ_CP066311:c224498-224100 [Azotobacter chroococcum]
MLDTCICSFIMRELPESVLQRLAAEVSQNNRIVISAVTYAEMRYGQIGKKASARHKTLVDEFVRRLDAVLPWDQRAVDAT
VEVMQALSKAGTPIGPNDTAIAGHAIASGCTLVTNNVREFGRVPGLVHEDWV

Nucleotide

• Length: 399 bp

>T184291 NZ_CP087606:330172-330540 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8513.66 Da
• Isoelectric Point: 5.0338

>AT184291 WP_040107521.1 NZ_CP066311:c224743-224513 [Azotobacter chroococcum]
MRTVSIFRNGKNQAIRLPNDMAYEGVGELEITREGDVITLRPVRPTWQSLAGVPKADADFLQERPTVVGDEGRFNL

Nucleotide

• Length: 231 bp

>AT184291 NZ_CP087606:329954-330175 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0C4WT59