Detailed information of TA system TA184289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 182182..183065
• Replicon: plasmid unnamed1
• Accession: NZ_CP066311
• Organism: Azotobacter chroococcum strain HR1

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A0C4WT88
• Locus tag: GKQ51_RS22780
• Protein ID: WP_040107448.1
• Coordinates: 182628..183065 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: PrcA
• Uniprot ID: -
• Locus tag: GKQ51_RS22775
• Protein ID: WP_089169991.1
• Coordinates: 182182..182631 (+)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKQ51_RS22750	177996..178196	-	201	Protein_190	transposase	-
GKQ51_RS22755	178313..179340	+	1028	Protein_191	IS630 family transposase	-
GKQ51_RS22760	179392..180330	-	939	WP_040107475.1	DMT family transporter	-
GKQ51_RS22765	180468..181184	-	717	WP_198868223.1	helix-turn-helix transcriptional regulator	-
GKQ51_RS22770	181381..181653	-	273	WP_165496622.1	hypothetical protein	-
GKQ51_RS22775	182182..182631	+	450	WP_089169991.1	DUF2384 domain-containing protein	Antitoxin
GKQ51_RS22780	182628..183065	+	438	WP_040107448.1	RES family NAD+ phosphorylase	Toxin
GKQ51_RS22785	183116..183298	+	183	WP_131254713.1	hypothetical protein	-
GKQ51_RS22790	183326..183721	+	396	WP_131254712.1	hypothetical protein	-
GKQ51_RS22795	184135..185034	-	900	WP_131254711.1	LysR family transcriptional regulator	-
GKQ51_RS22800	185143..185889	+	747	WP_136887957.1	SDR family oxidoreductase	-
GKQ51_RS22805	186012..187388	-	1377	WP_198866220.1	IS701 family transposase	-
GKQ51_RS22810	187468..187725	-	258	WP_090625628.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..307780	307780

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-140)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (97-143)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15880.25 Da
• Isoelectric Point: 5.1577

>T184289 WP_040107448.1 NZ_CP066311:182628-183065 [Azotobacter chroococcum]
VILWRISAYADLSGIGGLRASGRWHHLGRPVVYAADSPAGAMLEVLVHLEIDPEDFPTTLQLIRVELPDLVSRAELPPLP
EHWKSDPEVTRAMGDRFLDGRSALLLPVPSAIIPCTTNYLFNPAHPEASGASIQAEKFPLDSRLI

Nucleotide

• Length: 438 bp

>T184289 NZ_CP087598:c1665555-1665268 [Paracoccus sp. MA]
ATGAAGGCGCTGGCCTTCACCGTCGCGGCAACGGTCGATCTGGAGGCAATCTGGGACTATACGGAGGGCGAATGGGGCCT
CGCGCAAGCCCGGCGCTATGTGCAGGAACTGCGCGACACCTGCCACGCGCTGGCATCGGGGCAAGGGCGCGGCAGGCCGG
TTGACGTTCGGCCTGGCTATCTGAAATGCCTGAGCGGATCGCATGTCATCTTCTTCCGGGATCTGGGCGACACGCTGGAA
ATCGTCCGCATCCTGCACGGTGCACAGGACGTCGAGCGGCATTTGTGA

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 16966.54 Da
• Isoelectric Point: 6.7345

>AT184289 WP_089169991.1 NZ_CP066311:182182-182631 [Azotobacter chroococcum]
MLAEVLRDHGYTAYRARLQSLLDIPESATDFEIHTRITKGFPATRLVQLTEAGVVTPLERDQIIPLRTLKSRIERDQPLT
VEESDRFFRAAHITAMAEAVFGDREKAKRWLSKPKERFSGLTPMQMLTTQQGTSQVEEMLLQIVEGLAL

Nucleotide

• Length: 450 bp

>AT184289 NZ_CP087598:c1665797-1665552 [Paracoccus sp. MA]
ATGTCCCGCAACACATCGGTTTCGCTCGGCGATCACTTCGTCAGCTTCATCGACGCGCAGGTGAAGGGCGGCCGCTATGG
CTCGGCCAGCGACGTGGTGCGGGCAGGGCTGCGGCTCCTGGAGGAGCACGAGGCCAAGGTAAAGGCCCTTCAGGACGCCC
TGATCGCCGGAGAGGAATCCGGCCGGGCGGAGGGCTTCGACCTCGACCGGTTCATTGCCCGCAAGATGGCCGGTCGCAAC
GCATGA

Structures

Toxin

• AlphaFold DB: A0A0C4WT88