Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 139876..140494 Replicon plasmid unnamed1
Accession NZ_CP066311
Organism Azotobacter chroococcum strain HR1

Toxin (Protein)


Gene name hicA Uniprot ID A0A1I4HUC9
Locus tag GKQ51_RS22545 Protein ID WP_040107493.1
Coordinates 139876..140058 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0C4WXU9
Locus tag GKQ51_RS22550 Protein ID WP_040107336.1
Coordinates 140090..140494 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GKQ51_RS22515 135709..136422 - 714 WP_131300395.1 type 1 glutamine amidotransferase domain-containing protein -
GKQ51_RS22520 136521..137456 + 936 WP_198868214.1 LysR family transcriptional regulator -
GKQ51_RS22525 137492..137590 - 99 WP_089170148.1 IS66 family insertion sequence hypothetical protein -
GKQ51_RS22530 137626..137949 - 324 WP_136890245.1 transposase -
GKQ51_RS22535 138274..138531 + 258 Protein_147 IS4 family transposase -
GKQ51_RS22540 138568..139602 + 1035 WP_198868220.1 IS630 family transposase -
GKQ51_RS22545 139876..140058 + 183 WP_040107493.1 type II toxin-antitoxin system HicA family toxin Toxin
GKQ51_RS22550 140090..140494 + 405 WP_040107336.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GKQ51_RS22555 140620..140883 + 264 WP_040107494.1 hypothetical protein -
GKQ51_RS22560 141161..141761 + 601 Protein_152 cupin -
GKQ51_RS22565 141851..143125 - 1275 WP_198868215.1 OprD family porin -
GKQ51_RS22570 143290..144636 - 1347 WP_089169986.1 cytochrome c -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - adeG 1..307780 307780
- flank IS/Tn - - 137492..137629 137


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6800.85 Da        Isoelectric Point: 10.4803

>T184288 WP_040107493.1 NZ_CP066311:139876-140058 [Azotobacter chroococcum]
MRSREVIELLEADGWYEVAVKGSHHQFKHPTKPGRVTVPHPKSDLPKGTWQSILKQAGLK

Download         Length: 183 bp

>T184288 NZ_CP087598:1459969-1460403 [Paracoccus sp. MA]
ATGATCATCCTCGACACCAATGTCGTCTCGGAGACGATGAAGCCGGCGCCAGAGCCGCGGGTGATCGACTGGCTCAACCG
GCAAGAGTTGACCACCCTGCACCTGACCACCATCAGCCTGGCCGAGTTGCGGTTCGGCATTGCATGCCTGGACGCTGGGC
GGCGTCGGGACGATCTTGACGCGCGGCTCGAGCAGATGCTGGCCGAGGTATTTCCGGCACGTATCCTGTCGTTCGACGAG
GCCGCTGCCAGTGCCTTCGGAGTATTGATGGCAACGGCGCGCCGGCAGGGACAGGCCGTGAGTTTTGCCGATGGCGCGAT
TGCTGCCATTGCCGCGGCACAGGGCTATCCCGTCGCCAGCCGCGACACCGCGCCCTTCGCCGCCATGGGCGTGGAGGTGG
TGGATCCGTGGCACGAGGCGGCTCAAGGCCGGTAA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14695.64 Da        Isoelectric Point: 4.6292

>AT184288 WP_040107336.1 NZ_CP066311:140090-140494 [Azotobacter chroococcum]
MKYPVVIHKEQSSDYGVTVPDVPGCFSAGETMTEALENVREALELHFEGLLADGEPLPQPQEIDVHAANPDYAGGVWALV
DFDVMPYLGKAVRFNATLPEHLLARIDAYVKAHPEQKSRSGFLAEAALRVLQHA

Download         Length: 405 bp

>AT184288 NZ_CP087598:1459733-1459972 [Paracoccus sp. MA]
ATGGCCCAGATCGTCGTGCGTCAGATCCCGGAAGAGGTGCATCGCGCGCTGAAGGCGCAGGCCGCCGCGCATGGTCGCAG
CGCCGAGGCCGAGTTGCGCGAGATCATTGCCCGCGCGGTGCTGCCGCAGTCGCGCCCGCGCGCGGGTGATCTGATGCGCA
GTATCTGGTCCGGCGCCGAGACGGACGACCTTTCGGTCGAACGCGACCGGACGCCCGCGGAGCCGGCCGGTTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I4HUC9

Antitoxin

Source ID Structure
AlphaFold DB A0A0C4WXU9

References