Detailed information of TA system TA184287

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/Phd(antitoxin)
• Location: 111525..112123
• Replicon: plasmid unnamed1
• Accession: NZ_CP066311
• Organism: Azotobacter chroococcum strain HR1

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: GKQ51_RS22395
• Protein ID: WP_157731506.1
• Coordinates: 111782..112123 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0C4WSR8
• Locus tag: GKQ51_RS22390
• Protein ID: WP_040107312.1
• Coordinates: 111525..111785 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKQ51_RS22380	106610..108451	+	1842	WP_089169974.1	amidohydrolase	-
GKQ51_RS22385	108951..111143	+	2193	WP_089169975.1	catalase/peroxidase HPI	-
GKQ51_RS22390	111525..111785	+	261	WP_040107312.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GKQ51_RS22395	111782..112123	+	342	WP_157731506.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GKQ51_RS22400	112210..112509	+	300	WP_040107314.1	hypothetical protein	-
GKQ51_RS22405	112695..113450	-	756	WP_040107315.1	precorrin-4 C(11)-methyltransferase	-
GKQ51_RS22410	113447..113860	-	414	WP_040107316.1	cobalamin biosynthesis protein	-
GKQ51_RS22415	113880..114563	-	684	WP_040107317.1	CbtA family protein	-
GKQ51_RS22420	114571..114753	-	183	WP_040107318.1	CbtB-domain containing protein	-
GKQ51_RS22425	115443..116492	+	1050	WP_040107319.1	cobalamin biosynthesis protein CobW	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..307780	307780

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-99)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13111.96 Da
• Isoelectric Point: 5.1133

>T184287 WP_157731506.1 NZ_CP066311:111782-112123 [Azotobacter chroococcum]
MSFDVQFATTAETSLFSQIAHLEPYHSYDGAYEKLSTLVVEVIRRLADHPLGYPISAQVSELGIAHYRELNLNGYRVFYE
CDEEKRLVVVLLILGQRQSIEQQLIHYCLMFDR

Nucleotide

• Length: 342 bp

>T184287 NZ_CP087595:1085-1405 [Acinetobacter baumannii]
ATGTTATTCATTGAAACCAGTATTTTTACTAAGCAAATCAAAGAGCTTGTGAGTGATGAAGAGTATCGTCAGCTCCAGCA
AGATCTCTTGGTACAGCCTGATAAGGGCGACTTAATCAAGAATGGTGGTGGAATTCGTAAAGTACGTTGTGCTCAGGGCA
ATAAAGGCAAAAGTGGCGGTATCCGTGTGATCTATTATTGGGTTACAGAAGATGATCAGATCTTTTTCCTGGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAAACCTCGATTCTACGTCAATTAGTAAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9543.04 Da
• Isoelectric Point: 9.0005

>AT184287 WP_040107312.1 NZ_CP066311:111525-111785 [Azotobacter chroococcum]
MKIETISFLKKHAASLDLSEPMVITQNGLPAYVIESYAERKQRDETIALVKLLALGSRDKEQGKLVSGDTLLDRLAQRCQ
QPKGTS

Nucleotide

• Length: 261 bp

>AT184287 NZ_CP087595:1398-1670 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACCTGGTTGCTTCAATCAAAGAAGCTGGTGCCATCAAGCGTAAGGAGATAAAGGCAAG
TCGAGTCACAGAGCTTGAATTACCGGATATTAAAGAAGTACGTGAGAAAACAGGCTTAACCCAGGCTGAATTTGCTGCAC
GTTTGCATATCAGCGCGAGAACCCTGCAAAACTGGGAACAAGGCCGTCGCTATCCGACTGGTCCTGCAGCGACTTTAATT
CGTATTTTAGATGCTCATCCAACTTTAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0C4WSR8