Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | PfiT-PfiA/Phd(antitoxin) |
| Location | 63316..63914 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP066311 | ||
| Organism | Azotobacter chroococcum strain HR1 | ||
Toxin (Protein)
| Gene name | PfiT | Uniprot ID | - |
| Locus tag | GKQ51_RS22145 | Protein ID | WP_169531258.1 |
| Coordinates | 63573..63914 (+) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | PfiA | Uniprot ID | A0A0C4WLQ2 |
| Locus tag | GKQ51_RS22140 | Protein ID | WP_040107296.1 |
| Coordinates | 63316..63576 (+) | Length | 87 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GKQ51_RS22115 | 58971..60014 | + | 1044 | WP_040107291.1 | L-glyceraldehyde 3-phosphate reductase | - |
| GKQ51_RS22120 | 60017..60946 | + | 930 | WP_040107292.1 | aliphatic sulfonate ABC transporter substrate-binding protein | - |
| GKQ51_RS22125 | 61036..61884 | + | 849 | WP_198868200.1 | energy transducer TonB | - |
| GKQ51_RS22130 | 61918..62628 | + | 711 | WP_131300966.1 | MotA/TolQ/ExbB proton channel family protein | - |
| GKQ51_RS22135 | 62630..63031 | + | 402 | WP_040107295.1 | biopolymer transporter ExbD | - |
| GKQ51_RS22140 | 63316..63576 | + | 261 | WP_040107296.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| GKQ51_RS22145 | 63573..63914 | + | 342 | WP_169531258.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| GKQ51_RS22150 | 63911..64375 | + | 465 | WP_136888809.1 | hypothetical protein | - |
| GKQ51_RS22155 | 64479..64682 | + | 204 | WP_198868201.1 | hypothetical protein | - |
| GKQ51_RS22160 | 64848..65630 | + | 783 | WP_198868202.1 | TraI domain-containing protein | - |
| GKQ51_RS22165 | 65576..66040 | + | 465 | WP_198868218.1 | TraI domain-containing protein | - |
| GKQ51_RS22170 | 66195..66550 | + | 356 | Protein_74 | IS5/IS1182 family transposase | - |
| GKQ51_RS22175 | 66553..66714 | - | 162 | Protein_75 | DDE-type integrase/transposase/recombinase | - |
| GKQ51_RS22180 | 66843..67274 | - | 432 | WP_198868203.1 | IS200/IS605 family transposase | - |
| GKQ51_RS22185 | 67805..67983 | + | 179 | Protein_77 | helix-turn-helix domain-containing protein | - |
| GKQ51_RS22190 | 67983..68234 | + | 252 | Protein_78 | hypothetical protein | - |
| GKQ51_RS22195 | 68246..68419 | - | 174 | WP_169531021.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | adeG | 1..307780 | 307780 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 12941.71 Da Isoelectric Point: 5.5062
>T184286 WP_169531258.1 NZ_CP066311:63573-63914 [Azotobacter chroococcum]
MSFDVQFATTAETSLFSQIAHLEPYHGYDGAYGKLSTLVAEVIRRLADHPLGYPISAQASELGITHYRELNLDGYRVFYE
CDEEKRLVVVLLILGQRQSNEQQLIHYCLMFAR
MSFDVQFATTAETSLFSQIAHLEPYHGYDGAYGKLSTLVAEVIRRLADHPLGYPISAQASELGITHYRELNLDGYRVFYE
CDEEKRLVVVLLILGQRQSNEQQLIHYCLMFAR
Download Length: 342 bp
>T184286 NZ_CP087594:c3390505-3390116 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AATTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCACGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGTAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AATTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCACGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGTAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA
Antitoxin
Download Length: 87 a.a. Molecular weight: 9567.10 Da Isoelectric Point: 9.0005
>AT184286 WP_040107296.1 NZ_CP066311:63316-63576 [Azotobacter chroococcum]
MKIETISFLKKHAASLDLTEPMVITQNGLPAYVIESYAERKQRDETIALVKLLALGSRDKEQGKLVSGDTLLDRLAQRCQ
QPKGTP
MKIETISFLKKHAASLDLTEPMVITQNGLPAYVIESYAERKQRDETIALVKLLALGSRDKEQGKLVSGDTLLDRLAQRCQ
QPKGTP
Download Length: 261 bp
>AT184286 NZ_CP087594:c3390769-3390512 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|