Detailed information of TA system TA184284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 27338..28131
• Replicon: plasmid unnamed1
• Accession: NZ_CP066311
• Organism: Azotobacter chroococcum strain HR1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: GKQ51_RS21960
• Protein ID: WP_091014157.1
• Coordinates: 27338..27856 (-)
• Length: 173 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: GKQ51_RS21965
• Protein ID: WP_090943980.1
• Coordinates: 27856..28131 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKQ51_RS21925	22566..23234	+	669	WP_136890165.1	methyltransferase domain-containing protein	-
GKQ51_RS21930	23423..23958	+	536	Protein_26	endonuclease	-
GKQ51_RS21935	24004..25092	+	1089	WP_136890170.1	nitroreductase family protein	-
GKQ51_RS21940	25169..25465	+	297	WP_136890164.1	carboxymuconolactone decarboxylase family protein	-
GKQ51_RS21945	25620..26825	-	1206	WP_198866274.1	IS701 family transposase	-
GKQ51_RS21950	26966..27088	-	123	Protein_30	IS4 family transposase	-
GKQ51_RS21955	27098..27250	+	153	Protein_31	deoxyribonuclease I	-
GKQ51_RS21960	27338..27856	-	519	WP_091014157.1	GNAT family N-acetyltransferase	Toxin
GKQ51_RS21965	27856..28131	-	276	WP_090943980.1	DUF1778 domain-containing protein	Antitoxin
GKQ51_RS21970	28414..29307	-	894	WP_136890163.1	LysR family transcriptional regulator	-
GKQ51_RS21975	29420..30601	+	1182	WP_136890162.1	serine hydrolase	-
GKQ51_RS21980	30685..31863	+	1179	WP_136890169.1	MFS transporter	-
GKQ51_RS21985	31919..32953	+	1035	WP_198866948.1	IS630 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..307780	307780
	-	flank	IS/Tn	-	-	25620..26825	1205
	-	flank	IS/Tn	-	-	31826..32953	1127

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (9-85)

Sequences

Toxin

Protein

• Length: 173 a.a.
• Molecular weight: 18571.26 Da
• Isoelectric Point: 8.9007

>T184284 WP_091014157.1 NZ_CP066311:c27856-27338 [Azotobacter chroococcum]
MSLTAPHALDASHSLEGFDCGKPSLDDWLTRHARQAQASGSAKTYVVTDGVRVAGYYSLTVGQIDTLEAPARVRQGMGAY
PIPVVILARLAVAQRDQGLGIGVGLLQDAIRRTLTIAEQAGVRALLTHPIDEEASRFYLRFGFEASPAREQQLLLLLKDA
RRLLAPPTSPRG

Nucleotide

• Length: 519 bp

>T184284 NZ_CP087593:c2597280-2597014 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 9977.34 Da
• Isoelectric Point: 6.2499

>AT184284 WP_090943980.1 NZ_CP066311:c28131-27856 [Azotobacter chroococcum]
MASTTRSARLGLRATPQQEAVLRRAAEVANKSMTDFILDSACLAAEQTLLDQRLFLVSGSQSQALLDMLDRPAQDNPGLK
DLFSRSAPWDK

Nucleotide

• Length: 276 bp

>AT184284 NZ_CP087593:c2597537-2597280 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA