Detailed information of TA system TA184280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1730750..1731369
• Replicon: chromosome
• Accession: NZ_CP066310
• Organism: Azotobacter chroococcum strain HR1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: GKQ51_RS08075
• Protein ID: WP_131351239.1
• Coordinates: 1730750..1730932 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GKQ51_RS08080
• Protein ID: WP_131351236.1
• Coordinates: 1730962..1731369 (+)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKQ51_RS08055	1726078..1726302	-	225	WP_198867635.1	Arc family DNA-binding protein	-
GKQ51_RS08060	1726380..1727000	-	621	WP_198867636.1	hypothetical protein	-
GKQ51_RS08065	1727300..1728049	-	750	WP_089168903.1	IS5 family transposase	-
GKQ51_RS08070	1730295..1730552	-	258	WP_131351242.1	DUF1654 domain-containing protein	-
GKQ51_RS08075	1730750..1730932	+	183	WP_131351239.1	type II toxin-antitoxin system HicA family toxin	Toxin
GKQ51_RS08080	1730962..1731369	+	408	WP_131351236.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GKQ51_RS08085	1731397..1731648	-	252	WP_198867637.1	DUF1654 domain-containing protein	-
GKQ51_RS08090	1731821..1732096	+	276	WP_198867638.1	hypothetical protein	-
GKQ51_RS08095	1732133..1732621	-	489	WP_198867639.1	hypothetical protein	-
GKQ51_RS08100	1732820..1733197	+	378	WP_198867640.1	helix-turn-helix domain-containing protein	-
GKQ51_RS08105	1733198..1733743	+	546	WP_198867641.1	ImmA/IrrE family metallo-endopeptidase	-
GKQ51_RS08110	1734370..1735275	-	906	WP_198867642.1	endonuclease	-
GKQ51_RS08115	1735311..1735961	-	651	WP_131351216.1	helix-turn-helix transcriptional regulator	-
GKQ51_RS08120	1736049..1736255	+	207	WP_131351213.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1725196..1820868	95672
	-	flank	IS/Tn	-	-	1727300..1728049	749

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6602.74 Da
• Isoelectric Point: 10.7162

>T184280 WP_131351239.1 NZ_CP066310:1730750-1730932 [Azotobacter chroococcum]
VNSREVIGLIEADGWYLVRVNGSHHHFKHPTKPGLVTIPHPKKDLLAKTVGSILKQAGLK

Nucleotide

• Length: 183 bp

>T184280 NZ_CP066310:1730750-1730932 [Azotobacter chroococcum]
GTGAATAGCCGTGAAGTAATAGGCCTCATCGAGGCGGACGGCTGGTATCTGGTGCGCGTGAATGGCAGTCATCACCACTT
CAAGCACCCGACCAAGCCTGGGCTGGTCACCATCCCGCACCCGAAGAAGGATCTGCTGGCCAAGACAGTCGGCAGCATCT
TGAAACAGGCCGGACTCAAATAG

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14395.27 Da
• Isoelectric Point: 4.5885

>AT184280 WP_131351236.1 NZ_CP066310:1730962-1731369 [Azotobacter chroococcum]
MLYPIAISPGDEQHAWGVEVPDILGCFSAGDNLDDAMAMAREAIEGHLELLAEEGAQIPEASSVTTHASNPDYAGCTWAV
VDIDVTRYMGKAEKLNITLPGHLLARIDAYVKAHPEQKSRSGFLAAAALKALQHG

Nucleotide

• Length: 408 bp

>AT184280 NZ_CP066310:1730962-1731369 [Azotobacter chroococcum]
ATGCTTTATCCAATTGCAATCTCACCCGGCGACGAGCAGCACGCCTGGGGCGTCGAAGTGCCGGACATCCTCGGCTGTTT
CTCTGCGGGCGATAACCTGGACGATGCAATGGCGATGGCTCGCGAGGCCATCGAGGGGCATCTGGAACTGTTGGCCGAGG
AGGGCGCGCAGATCCCCGAGGCGAGCAGTGTGACCACGCATGCGAGCAATCCGGACTATGCCGGTTGCACCTGGGCGGTG
GTAGACATCGATGTGACCCGGTACATGGGCAAGGCAGAGAAACTGAACATCACGCTGCCGGGGCACTTGCTGGCCCGGAT
CGATGCCTATGTGAAGGCCCATCCGGAGCAGAAGAGCCGCTCCGGCTTCCTGGCTGCTGCAGCATTGAAGGCATTGCAGC
ACGGCTGA