Detailed information of TA system TA184277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 471248..471901
• Replicon: chromosome
• Accession: NZ_CP066308
• Organism: Brevibacillus sp. FJAT-54423

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JD108_RS02540
• Protein ID: WP_198828445.1
• Coordinates: 471551..471901 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: JD108_RS02535
• Protein ID: WP_198828444.1
• Coordinates: 471248..471547 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JD108_RS02500	466288..466623	+	336	WP_198828437.1	metalloregulator ArsR/SmtB family transcription factor	-
JD108_RS02505	466647..466898	-	252	WP_198828438.1	glutaredoxin family protein	-
JD108_RS02510	467097..467267	+	171	WP_198828439.1	gamma-type small acid-soluble spore protein	-
JD108_RS02515	467385..467783	+	399	WP_198828440.1	holo-ACP synthase	-
JD108_RS02520	468042..469076	+	1035	WP_198828441.1	DUF4367 domain-containing protein	-
JD108_RS02525	469440..469880	+	441	WP_198828442.1	hypothetical protein	-
JD108_RS02530	469884..471086	+	1203	WP_198828443.1	alanine racemase	-
JD108_RS02535	471248..471547	+	300	WP_198828444.1	CopG family ribbon-helix-helix protein	Antitoxin
JD108_RS02540	471551..471901	+	351	WP_198828445.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JD108_RS02545	472099..472521	+	423	WP_198828446.1	anti-sigma regulatory factor	-
JD108_RS02550	472530..473549	+	1020	WP_198828447.1	PP2C family protein-serine/threonine phosphatase	-
JD108_RS02555	473551..473877	+	327	WP_198828448.1	STAS domain-containing protein	-
JD108_RS02560	473877..474365	+	489	WP_198828449.1	anti-sigma B factor RsbW	-
JD108_RS02565	474334..475122	+	789	WP_198828450.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (15-52)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12961.98 Da
• Isoelectric Point: 4.8553

>T184277 WP_198828445.1 NZ_CP066308:471551-471901 [Brevibacillus sp. FJAT-54423]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKMYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMERVNESLQISLGIIDF

Nucleotide

• Length: 351 bp

>T184277 NZ_CP087593:c2099232-2099056 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11288.17 Da
• Isoelectric Point: 10.2102

>AT184277 WP_198828444.1 NZ_CP066308:471248-471547 [Brevibacillus sp. FJAT-54423]
MVGGGFVLSKSNTKRIMISLPHHLLQEVDGVIQKEKSNRSEFIRQAMHLYLKERKKRAIRETMQKGYMEMAKINLNMASE
AFGAEEEADHTLVRLVSGV

Nucleotide

• Length: 300 bp

>AT184277 NZ_CP087593:2098933-2098988 [Staphylococcus aureus]
AAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG