Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 57827..58520 Replicon plasmid pBR-PH17
Accession NZ_CP066307
Organism Pseudomonas putida strain BR-PH17

Toxin (Protein)


Gene name tad Uniprot ID N2IHR9
Locus tag JET17_RS27015 Protein ID WP_003151133.1
Coordinates 58143..58520 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID N2IIN5
Locus tag JET17_RS27010 Protein ID WP_001172026.1
Coordinates 57827..58162 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JET17_RS26985 53126..56080 + 2955 WP_015272413.1 conjugative relaxase -
JET17_RS26990 56116..56625 - 510 WP_015272414.1 hypothetical protein -
JET17_RS26995 56770..57111 - 342 WP_025999701.1 hypothetical protein -
JET17_RS27000 57127..57453 - 327 WP_000091614.1 hypothetical protein -
JET17_RS27005 57477..57812 - 336 WP_000741275.1 hypothetical protein -
JET17_RS27010 57827..58162 - 336 WP_001172026.1 helix-turn-helix domain-containing protein Antitoxin
JET17_RS27015 58143..58520 - 378 WP_003151133.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JET17_RS27020 58712..59314 + 603 WP_010465829.1 recombinase family protein -
JET17_RS27025 59298..62327 + 3030 WP_010799689.1 Tn3 family transposase -
JET17_RS27030 62469..63353 + 885 WP_074241320.1 class A beta-lactamase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul1 / tet(A) / sul2 / aph(6)-Id / aph(3'')-Ib / aph(3')-Ia / qacE / cmlA1 / ant(3'')-Ia / blaVIM-1 / blaAER-1 / ant(2'')-Ia - 1..80360 80360


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-93)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13705.78 Da        Isoelectric Point: 9.4693

>T184276 WP_003151133.1 NZ_CP066307:c58520-58143 [Pseudomonas putida]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Download         Length: 378 bp

>T184276 NZ_CP087593:c1952809-1952657 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12201.39 Da        Isoelectric Point: 10.6058

>AT184276 WP_001172026.1 NZ_CP066307:c58162-57827 [Pseudomonas putida]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Download         Length: 336 bp

>AT184276 NZ_CP087593:c1953433-1952834 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A024ELN5

Antitoxin

Source ID Structure
AlphaFold DB A0A024EKI7

References