Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 42319..42989 Replicon plasmid pBR-PH17
Accession NZ_CP066307
Organism Pseudomonas putida strain BR-PH17

Toxin (Protein)


Gene name vagD Uniprot ID Q2QCR9
Locus tag JET17_RS26900 Protein ID WP_012477632.1
Coordinates 42552..42989 (+) Length 146 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q2QCS0
Locus tag JET17_RS26895 Protein ID WP_012477631.1
Coordinates 42319..42555 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JET17_RS26865 37826..38032 + 207 WP_015272391.1 zinc ribbon domain-containing protein -
JET17_RS26870 38025..38642 + 618 WP_015272392.1 recombinase family protein -
JET17_RS26875 38629..39765 + 1137 WP_015272393.1 replication initiator protein A -
JET17_RS26880 40204..40416 - 213 WP_015272394.1 hypothetical protein -
JET17_RS26885 40611..41261 + 651 WP_015272395.1 DNA-binding protein -
JET17_RS26890 41884..42309 + 426 WP_015272397.1 DUF192 domain-containing protein -
JET17_RS26895 42319..42555 + 237 WP_012477631.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JET17_RS26900 42552..42989 + 438 WP_012477632.1 type II toxin-antitoxin system VapC family toxin Toxin
JET17_RS26905 42996..43157 + 162 WP_015272398.1 hypothetical protein -
JET17_RS26910 43285..43545 + 261 WP_015272399.1 hypothetical protein -
JET17_RS26915 44226..45062 + 837 WP_015272400.1 DUF945 domain-containing protein -
JET17_RS26920 45118..45546 + 429 WP_015272401.1 hypothetical protein -
JET17_RS26925 45563..46066 + 504 WP_015272402.1 hypothetical protein -
JET17_RS26930 46127..46477 + 351 WP_015272403.1 hypothetical protein -
JET17_RS26935 46525..46737 + 213 WP_153334493.1 hypothetical protein -
JET17_RS26940 47395..47649 + 255 WP_015272405.1 hypothetical protein -
JET17_RS26945 47720..47896 + 177 WP_019692578.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul1 / tet(A) / sul2 / aph(6)-Id / aph(3'')-Ib / aph(3')-Ia / qacE / cmlA1 / ant(3'')-Ia / blaVIM-1 / blaAER-1 / ant(2'')-Ia - 1..80360 80360
- flank IS/Tn sul1 / qacE / cmlA1 / ant(3'')-Ia / blaVIM-1 - 29026..37735 8709


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15671.07 Da        Isoelectric Point: 8.5199

>T184275 WP_012477632.1 NZ_CP066307:42552-42989 [Pseudomonas putida]
MSKKTYMLDTNICSFIMRERPDAVLAKLEQAVTNQHRIVVSAITYSEMRFGAANPKASPKVAAMVDAFIQRLDAILSWDA
AAVDQTTQIRTALARLGTPIGNNDAAIAGHALAAGCVLVTNNTREFARVPGLVLEDWTQPAITKS

Download         Length: 438 bp

>T184275 NZ_CP087593:1894798-1894893 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 9070.29 Da        Isoelectric Point: 4.4563

>AT184275 WP_012477631.1 NZ_CP066307:42319-42555 [Pseudomonas putida]
MRTVSIFMNSRNQAIRIPRDMEYQGVSELEIIRDGETLILRPVRPSWASLRDVAAADPDFLTERKDVIEEDRFLGDFT

Download         Length: 237 bp

>AT184275 NZ_CP087593:c1894980-1894921 [Staphylococcus aureus]
ACAACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A010RDD2

Antitoxin

Source ID Structure
AlphaFold DB A0A010SZE6

References