184274

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 18130..18823 Replicon plasmid pBR-PH17
Accession NZ_CP066307
Organism Pseudomonas putida strain BR-PH17

Toxin (Protein)


Gene name tad Uniprot ID N2IHR9
Locus tag JET17_RS26760 Protein ID WP_003151133.1
Coordinates 18446..18823 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID N2IIN5
Locus tag JET17_RS26755 Protein ID WP_001172026.1
Coordinates 18130..18465 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JET17_RS26715 13790..14593 - 804 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib -
JET17_RS26720 14659..14979 - 321 Protein_15 recombinase family protein -
JET17_RS26725 15033..15737 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
JET17_RS26730 15894..16709 - 816 WP_000018326.1 aminoglycoside O-phosphotransferase APH(3')-Ia -
JET17_RS26735 16860..17033 + 174 Protein_18 IS6 family transposase -
JET17_RS26740 17073..17414 - 342 WP_025999701.1 hypothetical protein -
JET17_RS26745 17430..17756 - 327 WP_000091614.1 hypothetical protein -
JET17_RS26750 17780..18115 - 336 WP_000741275.1 hypothetical protein -
JET17_RS26755 18130..18465 - 336 WP_001172026.1 helix-turn-helix domain-containing protein Antitoxin
JET17_RS26760 18446..18823 - 378 WP_003151133.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JET17_RS26765 19015..19617 + 603 WP_010465829.1 recombinase family protein -
JET17_RS26770 19601..22630 + 3030 WP_010799689.1 Tn3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn sul1 / tet(A) / sul2 / aph(6)-Id / aph(3'')-Ib / aph(3')-Ia - 1740..22630 20890
- inside Mobilizable plasmid sul1 / tet(A) / sul2 / aph(6)-Id / aph(3'')-Ib / aph(3')-Ia / qacE / cmlA1 / ant(3'')-Ia / blaVIM-1 / blaAER-1 / ant(2'')-Ia - 1..80360 80360


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-93)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13705.78 Da        Isoelectric Point: 9.4693

>T184274 WP_003151133.1 NZ_CP066307:c18823-18446 [Pseudomonas putida]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Download         Length: 378 bp

>T184274 NZ_CP087593:c865311-865123 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTAGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTATCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12201.39 Da        Isoelectric Point: 10.6058

>AT184274 WP_001172026.1 NZ_CP066307:c18465-18130 [Pseudomonas putida]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Download         Length: 336 bp

>AT184274 NZ_CP087593:c865600-865340 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A024ELN5

Antitoxin

Source ID Structure
AlphaFold DB A0A024EKI7

References