Detailed information of TA system TA184273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5109663..5110303
• Replicon: chromosome
• Accession: NZ_CP066306
• Organism: Pseudomonas putida strain BR-PH17

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JET17_RS23560
• Protein ID: WP_012316423.1
• Coordinates: 5109893..5110303 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A427FTN1
• Locus tag: JET17_RS23555
• Protein ID: WP_012316422.1
• Coordinates: 5109663..5109893 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JET17_RS23535	5105050..5106381	-	1332	WP_012316418.1	precorrin-3B synthase	-
JET17_RS23540	5106557..5107768	+	1212	WP_042111775.1	bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase	-
JET17_RS23545	5107761..5108855	+	1095	WP_012316420.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
JET17_RS23550	5108852..5109571	+	720	WP_012316421.1	cobalt-precorrin-6A reductase	-
JET17_RS23555	5109663..5109893	+	231	WP_012316422.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JET17_RS23560	5109893..5110303	+	411	WP_012316423.1	tRNA(fMet)-specific endonuclease VapC	Toxin
JET17_RS23565	5110438..5110875	+	438	WP_012316424.1	NfeD family protein	-
JET17_RS23570	5110900..5111754	+	855	WP_012316425.1	SPFH/Band 7/PHB domain protein	-
JET17_RS23575	5111948..5112337	+	390	WP_012316426.1	DUF2946 domain-containing protein	-
JET17_RS23580	5112386..5112865	+	480	WP_012316427.1	copper chaperone PCu(A)C	-
JET17_RS23585	5112892..5113323	+	432	WP_012316428.1	DUF2946 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15055.42 Da
• Isoelectric Point: 8.0998

>T184273 WP_012316423.1 NZ_CP066306:5109893-5110303 [Pseudomonas putida]
MLKYMLDTNICIFTIKNKPQVVREAFNRHHGQLAISTVTLMELVYGAEKSAAPARNLSIVEGFVARLEVLDYDSHAAEHS
GQLRSELAKAGTPIGPFDQLIAGHARARGLTLVTNNLREFMRVPGLRVEDWLAMPG

Nucleotide

• Length: 411 bp

>T184273 NZ_CP087593:c323742-323278 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTAGA
CGGGGTATGGTTAGGAGATTTAATTTTGATAAAGCGTGGTTTATCAGATAGAGAAAAAGCAGGAATTCTCTTCGAAGAAT
TAGCGCATAATAAACTTACATACGGTGATATAGCCGATTACTCGAATTTCAACAATCGCAAGTTCGAAAATTACGCAAGA
CGACACGGCTTTATCTCAGCAGTCCCGTTACGCGAAATTGTGGAAGCGCACAATTACGGTGTACGTAACTTGTATGAGTT
GTCTGAGTATCTACAATTAAGTGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGTACTC
ACTACGGCGAATTCTCAATTACATTTGAGCCGTTGAGAGTTTTTAAATTGCATCATATTGATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8487.35 Da
• Isoelectric Point: 4.2821

>AT184273 WP_012316422.1 NZ_CP066306:5109663-5109893 [Pseudomonas putida]
MEQGAVFKSNRSQAIRLPKSVAFPEEVTRVDIVAVGRTRIITPAGESWDSWFDGEDASPDFMASRDQPADQEREGF

Nucleotide

• Length: 231 bp

>AT184273 NZ_CP087593:c324069-323755 [Staphylococcus aureus]
ATGTGCTTTTCAAAAAGAATGAAACAATCAAGAGAAAAACAAGGTATGACTTTGGCCGAACTAGGAAGAAAAATTGGTAA
AACTGAAGCTACTGTACAACGTTATGAAAGCGGAAATATCAAAAATCTAAAAAACGATACTATAGAAAGTATAGCTACTG
CATTAAATGTTAATCCTGCGTATTTAATGGGGTGGGTTGAAGAAAACGATGATGAAGTACAACATCGTGCAGCTCATTTA
GAAGGAGAATTAACTGATGACGAGTGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGTAAACGTAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A427FTN1