Detailed information of TA system TA184263

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 12570..13017
• Replicon: plasmid p2
• Accession: NZ_CP066272
• Organism: Pseudomonas tremae strain PA-1-10F

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A3M4C5F0
• Locus tag: I9H09_RS26540
• Protein ID: WP_003348606.1
• Coordinates: 12745..13017 (+)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A3M4C5H0
• Locus tag: I9H09_RS26535
• Protein ID: WP_005730485.1
• Coordinates: 12570..12755 (+)
• Length: 62 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9H09_RS26500 (I9H09_26360)	7841..7930	+	90	WP_231994119.1	DUF4113 domain-containing protein	-
I9H09_RS26505 (I9H09_26365)	8299..8697	-	399	Protein_9	transposase	-
I9H09_RS26510 (I9H09_26370)	8882..9370	-	489	WP_024421281.1	hypothetical protein	-
I9H09_RS26515 (I9H09_26375)	9700..10380	+	681	WP_005730279.1	AAA family ATPase	-
I9H09_RS26520 (I9H09_26380)	10371..10733	+	363	WP_005730278.1	hypothetical protein	-
I9H09_RS26525 (I9H09_26385)	11513..12148	+	636	WP_103724617.1	recombinase family protein	-
I9H09_RS26530 (I9H09_26390)	12241..12519	+	279	WP_003320513.1	hypothetical protein	-
I9H09_RS26535 (I9H09_26395)	12570..12755	+	186	WP_005730485.1	hypothetical protein	Antitoxin
I9H09_RS26540 (I9H09_26400)	12745..13017	+	273	WP_003348606.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I9H09_RS26545 (I9H09_26405)	13121..13834	+	714	WP_044311048.1	YecA family protein	-
I9H09_RS26550 (I9H09_26410)	13879..14212	+	334	Protein_18	transposase	-
I9H09_RS26555 (I9H09_26415)	14652..15206	+	555	WP_031598886.1	hypothetical protein	-
I9H09_RS26560 (I9H09_26420)	15257..15487	+	231	WP_031598890.1	carbon storage regulator	-
I9H09_RS26565	15605..15718	-	114	Protein_21	resolvase	-
I9H09_RS26570 (I9H09_26425)	16022..16546	-	525	WP_080719055.1	DUF2778 domain-containing protein	-
I9H09_RS26575 (I9H09_26430)	16570..17070	-	501	WP_236502550.1	type VI secretion system tube protein Hcp	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..71198	71198
	-	flank	IS/Tn	-	-	13946..14212	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-89)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10199.67 Da
• Isoelectric Point: 5.0749

>T184263 WP_003348606.1 NZ_CP066272:12745-13017 [Pseudomonas tremae]
VLVEWLPQALEALEEIAGYLAERNPYAAEQMQATLEATVEALPLHPYIHRPGRVPGTREAVVHPNYLVVYRVGTERISIL
DVLHARQEYP

Nucleotide

• Length: 273 bp

>T184263 NZ_CP087584:c881016-880813 [Providencia rettgeri]
ATGACCAAATCTGATTATCTGATGCGTTTGAGAAAATGCACAACAATTGAAACACTTGAGCGTGTCATTGAAAAAAACAA
GTATGAACTATCTGATGATGAACTAGAGTTATTCTACTCAGCGGCAGATCATCGTCTTGCTGAACTCACTATGAATAAGC
TCTACGATAAAATTCCACCTTCTGTATGGAAGTTTGTTAGATAA

Antitoxin

Protein

• Length: 62 a.a.
• Molecular weight: 7292.18 Da
• Isoelectric Point: 7.2040

>AT184263 WP_005730485.1 NZ_CP066272:12570-12755 [Pseudomonas tremae]
MSIQLDPRVSEFETQEQADNYDRWFRQRIEHSLADPRPPVPHDEAMARVRAMIEAKRRRAS

Nucleotide

• Length: 186 bp

>AT184263 NZ_CP087584:c881459-881091 [Providencia rettgeri]
ATGGATGAATACTCACCCAAAAGACATGATATAGCAGAACTAAAATACTTATGTAACAGTCTCAATAGAGACGCTATCTT
AAGTTTGCAAAAAACCAACACACATTGGATTAACGATCTAAGCTCTGCACAAAGTGCAGCTCTAAATGAACTCATTGAAC
ATATCGCAGCTTTTGTCTGGCGATTTAAGATCAAATATCCAAAAGAAAATCTCGTTATATCATTAATTGAAGAGTACCTC
GATGAGACTTATGACTTATTTGGTAGCCCAGTCATCACATTAAGTGAAATTATAGATTGGGAAACTATGAATCAAAATCT
AGTTTCAGTGCTGGATGATGACCTAAAATGTCTGACCAGCAAAACATAA

Structures

Toxin

• AlphaFold DB: A0A3M4C5F0

Antitoxin

• AlphaFold DB: A0A3M4C5H0