Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE-Phd
Location 66928..67539 Replicon plasmid p1
Accession NZ_CP066271
Organism Pseudomonas tremae strain PA-1-10F

Toxin (Protein)


Gene name PfiT Uniprot ID A0A0P9J3E0
Locus tag I9H09_RS26260 Protein ID WP_054990073.1
Coordinates 67189..67539 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0P9IR64
Locus tag I9H09_RS26255 Protein ID WP_054990072.1
Coordinates 66928..67176 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I9H09_RS26225 (I9H09_26105) 62305..63066 + 762 WP_081032889.1 alpha/beta fold hydrolase -
I9H09_RS26230 (I9H09_26110) 63072..63710 + 639 WP_054990069.1 alanyl-tRNA editing protein -
I9H09_RS26235 (I9H09_26115) 63729..64340 + 612 WP_054990070.1 hypothetical protein -
I9H09_RS26240 (I9H09_26120) 64493..64996 - 504 Protein_58 IS5 family transposase -
I9H09_RS26245 (I9H09_26125) 65258..65518 + 261 WP_122346902.1 hypothetical protein -
I9H09_RS26250 (I9H09_26130) 65644..66606 - 963 WP_054990071.1 tyrosine-type recombinase/integrase -
I9H09_RS26255 (I9H09_26135) 66928..67176 + 249 WP_054990072.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
I9H09_RS26260 (I9H09_26140) 67189..67539 + 351 WP_054990073.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I9H09_RS26265 (I9H09_26145) 67956..68180 - 225 Protein_63 IS630 family transposase -
I9H09_RS26275 (I9H09_26155) 69417..70268 - 852 Protein_65 IS630-like element ISPsy25 family transposase -
I9H09_RS26280 (I9H09_26160) 70577..70741 - 165 WP_081032891.1 rubredoxin -
I9H09_RS26285 (I9H09_26165) 70763..71101 - 339 WP_081032892.1 hypothetical protein -
I9H09_RS26290 (I9H09_26170) 71119..71832 - 714 WP_234778524.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..98897 98897
- inside IScluster/Tn - - 64493..70268 5775


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-101)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12949.83 Da        Isoelectric Point: 4.4192

>T184261 WP_054990073.1 NZ_CP066271:67189-67539 [Pseudomonas tremae]
MNTFALRFTDVAQQSLEDQVEHLAVHQGFSSAAQRIDSLIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMDSEGITVIVVLLVLGGKQSVEQALIRYCLLQPI

Download         Length: 351 bp

>T184261 NZ_CP087580:3933027-3933245 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9378.78 Da        Isoelectric Point: 9.8450

>AT184261 WP_054990072.1 NZ_CP066271:66928-67176 [Pseudomonas tremae]
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYAQGKHCSTDELKARLSRRFI
QK

Download         Length: 249 bp

>AT184261 NZ_CP087580:3932626-3933000 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0P9J3E0

Antitoxin

Source ID Structure
AlphaFold DB A0A0P9IR64

References