184260

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 71684..72258 Replicon plasmid p1
Accession NZ_CP066268
Organism Pseudomonas tremae strain PA-1-12B

Toxin (Protein)


Gene name vapC Uniprot ID Q48BC5
Locus tag I9H06_RS27055 Protein ID WP_003344395.1
Coordinates 71684..72061 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q48BC4
Locus tag I9H06_RS27060 Protein ID WP_004644067.1
Coordinates 72058..72258 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I9H06_RS27035 (I9H06_26900) 67326..69410 + 2085 WP_248943784.1 DotA/TraY family protein -
I9H06_RS27040 (I9H06_26905) 69513..69758 + 246 WP_003344389.1 hypothetical protein -
I9H06_RS27045 (I9H06_26910) 69755..70399 + 645 WP_005769751.1 plasmid IncI1-type surface exclusion protein ExcA -
I9H06_RS27050 (I9H06_26915) 70492..71694 + 1203 WP_236534705.1 conjugal transfer protein -
I9H06_RS27055 (I9H06_26920) 71684..72061 - 378 WP_003344395.1 PIN domain-containing protein Toxin
I9H06_RS27060 (I9H06_26925) 72058..72258 - 201 WP_004644067.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
I9H06_RS27065 (I9H06_26930) 72350..73435 + 1086 WP_122324283.1 thioredoxin fold domain-containing protein -
I9H06_RS27070 (I9H06_26935) 73422..75710 + 2289 WP_057429711.1 F-type conjugative transfer protein TrbC -
I9H06_RS27075 (I9H06_26940) 75738..76160 + 423 WP_248943781.1 hypothetical protein -
I9H06_RS27080 (I9H06_26945) 76181..76744 + 564 WP_099264719.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..78055 78055


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-43)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13633.73 Da        Isoelectric Point: 6.4723

>T184260 WP_003344395.1 NZ_CP066268:c72061-71684 [Pseudomonas tremae]
VKGVLVDTSVWVEHFRNNSPELVNLLSQDRVLIHPMVIGELACGTPPDRSNTLTDLGDLRGAQQPTVSEVIAFLNTHKLY
GLGCGLVDMTLLASALLSGTALWTLDKRLERLASRMAVSYQPPTH

Download         Length: 378 bp

>T184260 NZ_CP087580:c3788622-3788305 [Klebsiella quasipneumoniae]
ATGTTCAGAATGGTTGTGCATGTGGACGTTAAAAAGGAGTTGCAGGCGCTTCCGGCTATTGTCCAGGCGAAGATGATCAG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGATAGTAAACCGCTTGGACAAGGGCTATTTGAAA
TTCGAGCGTTAGGTTCGGTACAGGGGCGGGGGATCTACGTTTTTCAGCAAAACAAAACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTTATCGGAAATTCGCTTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7445.66 Da        Isoelectric Point: 7.0102

>AT184260 WP_004644067.1 NZ_CP066268:c72258-72058 [Pseudomonas tremae]
MRTTVTIDDALYQRALEVADPAMDKADLFREAVQTFVRIQAAKRLMALGATLPTMEDIARRHEKAL

Download         Length: 201 bp

>AT184260 NZ_CP087580:c3788312-3788025 [Klebsiella quasipneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAGCACCGATTACGTACTATGCTGGCACAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCACCAGCCTGGATACCTTAACGCGA
TACGCGTTAGCGTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3GPE2

Antitoxin

Source ID Structure
AlphaFold DB A0A2S4IKB9

References