Detailed information of TA system TA184256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1343151..1343773
• Replicon: chromosome
• Accession: NZ_CP066265
• Organism: Pseudomonas syringae strain PA-2-9E

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: I9H07_RS06105
• Protein ID: WP_024672261.1
• Coordinates: 1343151..1343333 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I9H07_RS06110
• Protein ID: WP_024672260.1
• Coordinates: 1343369..1343773 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9H07_RS06095 (I9H07_06035)	1339351..1341099	-	1749	WP_236423636.1	N-acetylglutaminylglutamine synthetase	-
I9H07_RS06100 (I9H07_06040)	1341103..1342875	-	1773	WP_024672262.1	N-acetylglutaminylglutamine amidotransferase	-
I9H07_RS06105 (I9H07_06045)	1343151..1343333	+	183	WP_024672261.1	type II toxin-antitoxin system HicA family toxin	Toxin
I9H07_RS06110 (I9H07_06050)	1343369..1343773	+	405	WP_024672260.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
I9H07_RS06115 (I9H07_06055)	1343789..1344868	-	1080	WP_024672259.1	lipocalin-like domain-containing protein	-
I9H07_RS06120 (I9H07_06060)	1344858..1347338	-	2481	WP_058823921.1	ABC transporter permease	-
I9H07_RS06125 (I9H07_06065)	1347340..1348008	-	669	WP_024672257.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6750.00 Da
• Isoelectric Point: 11.1589

>T184256 WP_024672261.1 NZ_CP066265:1343151-1343333 [Pseudomonas syringae]
VQSRHLMKELEAHGWILDRVTGSHHMFKHPQKAQTVPVPHPKKDLPVGTVKAIRKLAGLV

Nucleotide

• Length: 183 bp

>T184256 NZ_CP087579:5852-6157 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14545.71 Da
• Isoelectric Point: 5.5902

>AT184256 WP_024672260.1 NZ_CP066265:1343369-1343773 [Pseudomonas syringae]
MKYPMCIEWGDETTAFGIQIPDIPGAISAGDTFEQAHAAAVEIAHIMLQEIAASGGTLPKARTVAEHAKNPDFSGMGWGM
LEIDITLYLGKTEKVNVTLPGFVIKQIDRYVRDHSIKSRSTFLADAALEKLGRA

Nucleotide

• Length: 405 bp

>AT184256 NZ_CP087579:5632-5850 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA