Detailed information of TA system TA184254

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 4481428..4481953
• Replicon: chromosome
• Accession: NZ_CP066263
• Organism: Pseudomonas syringae pv. syringae strain Pss9644

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: I9H08_RS18870
• Protein ID: WP_103359332.1
• Coordinates: 4481663..4481953 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q4ZPR0
• Locus tag: I9H08_RS18865
• Protein ID: WP_003402372.1
• Coordinates: 4481428..4481673 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9H08_RS18860 (I9H08_18800)	4477343..4481254	-	3912	WP_024640258.1	ATP-dependent RNA helicase HrpA	-
I9H08_RS18865 (I9H08_18805)	4481428..4481673	+	246	WP_003402372.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
I9H08_RS18870 (I9H08_18810)	4481663..4481953	+	291	WP_103359332.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I9H08_RS18875 (I9H08_18815)	4482271..4483962	-	1692	WP_011268682.1	long-chain-fatty-acid--CoA ligase FadD1	-
I9H08_RS18880 (I9H08_18820)	4484318..4486006	-	1689	WP_024640260.1	long-chain-fatty-acid--CoA ligase FadD2	-
I9H08_RS18885 (I9H08_18825)	4486375..4486845	+	471	WP_003402365.1	MaoC family dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-71)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10995.98 Da
• Isoelectric Point: 10.7294

>T184254 WP_103359332.1 NZ_CP066263:4481663-4481953 [Pseudomonas syringae pv. syringae]
MTFKLEFLPSALKEWEKLGHTIRVQLKKKLLERLGLPRIPGDALHGMPDHYKIKLRSAGYRLVYRVEDDRVVVTVVAVGK
RERGNIYDSAKGRLGP

Nucleotide

• Length: 291 bp

>T184254 NZ_CP087578:c4491975-4491448 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGAATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8827.22 Da
• Isoelectric Point: 4.5349

>AT184254 WP_003402372.1 NZ_CP066263:4481428-4481673 [Pseudomonas syringae pv. syringae]
MQSILADVAVSVSELKKNPSAVLSGAHGLPVAVLNHNKVMGYMVPAEVFEAMMERLDDLDLAELIKSRQHEKPVSVDIDD
L

Nucleotide

• Length: 246 bp

>AT184254 NZ_CP087578:c4492248-4491982 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3M2V2W1