Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 102974..103617 Replicon plasmid p3
Accession NZ_CP066256
Organism Klebsiella pneumoniae strain CRKP78R

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag JCR35_RS28380 Protein ID WP_001044770.1
Coordinates 102974..103390 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag JCR35_RS28385 Protein ID WP_001261282.1
Coordinates 103387..103617 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCR35_RS28360 99077..99349 - 273 Protein_121 transposase -
JCR35_RS28365 99378..100075 - 698 Protein_122 IS1 family transposase -
JCR35_RS28370 100331..101353 - 1023 WP_000361404.1 helicase UvrD -
JCR35_RS28375 101338..102900 - 1563 WP_004206609.1 AAA family ATPase -
JCR35_RS28380 102974..103390 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
JCR35_RS28385 103387..103617 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JCR35_RS28390 103574..104035 + 462 WP_014343465.1 hypothetical protein -
JCR35_RS28395 104196..105140 + 945 WP_011977810.1 hypothetical protein -
JCR35_RS28400 105177..105569 + 393 WP_011977811.1 hypothetical protein -
JCR35_RS28405 105627..106148 + 522 WP_013214008.1 hypothetical protein -
JCR35_RS28410 106194..106397 + 204 WP_011977813.1 hypothetical protein -
JCR35_RS28415 106427..107431 + 1005 WP_011977814.1 hypothetical protein -
JCR35_RS28420 107615..108394 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-65 / blaSHV-12 / rmtB / blaTEM-1B / fosA3 / blaKPC-2 - 1..149407 149407


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T184251 WP_001044770.1 NZ_CP066256:c103390-102974 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T184251 NZ_CP087578:c3786588-3786253 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT184251 WP_001261282.1 NZ_CP066256:c103617-103387 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT184251 NZ_CP087578:c3786836-3786588 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References