Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5324286..5324911 Replicon chromosome
Accession NZ_CP066254
Organism Klebsiella pneumoniae strain CRKP78R

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JCR35_RS26230 Protein ID WP_002882817.1
Coordinates 5324286..5324669 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JCR35_RS26235 Protein ID WP_004150355.1
Coordinates 5324669..5324911 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCR35_RS26215 5321652..5322554 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JCR35_RS26220 5322551..5323186 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JCR35_RS26225 5323183..5324112 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JCR35_RS26230 5324286..5324669 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JCR35_RS26235 5324669..5324911 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JCR35_RS26240 5325116..5326033 + 918 WP_002882812.1 alpha/beta hydrolase -
JCR35_RS26245 5326047..5326988 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JCR35_RS26250 5327033..5327470 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JCR35_RS26255 5327467..5328327 - 861 WP_002882807.1 virulence factor BrkB family protein -
JCR35_RS26260 5328321..5328920 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T184243 WP_002882817.1 NZ_CP066254:c5324669-5324286 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T184243 NZ_CP087578:2356564-2356740 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT184243 WP_004150355.1 NZ_CP066254:c5324911-5324669 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT184243 NZ_CP087578:2356786-2357202 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References