Detailed information of TA system TA184225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 52738..53318
• Replicon: plasmid pCRKP52R-4-tetA
• Accession: NZ_CP066252
• Organism: Klebsiella pneumoniae strain CRKP52R

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: JCR34_RS29135
• Protein ID: WP_071177730.1
• Coordinates: 53004..53318 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: JCR34_RS29130
• Protein ID: WP_000093040.1
• Coordinates: 52738..53016 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCR34_RS29100	47897..48676	-	780	WP_108970993.1	Y-family DNA polymerase	-
JCR34_RS29105	48676..49101	-	426	WP_045325066.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
JCR34_RS29110	49179..50666	-	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
JCR34_RS29115	51311..51556	+	246	WP_032440458.1	hypothetical protein	-
JCR34_RS29120	51830..52201	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
JCR34_RS29125	52198..52404	+	207	Protein_67	TonB family protein	-
JCR34_RS29130	52738..53016	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
JCR34_RS29135	53004..53318	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JCR34_RS29140	53482..53910	+	429	WP_001140599.1	hypothetical protein	-
JCR34_RS29145	53936..54115	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
JCR34_RS29150	54142..54672	-	531	WP_071177729.1	hypothetical protein	-
JCR34_RS29155	54679..55410	-	732	WP_071177728.1	replication-relaxation family protein	-
JCR34_RS29160	55410..57374	-	1965	WP_071177727.1	TraM recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrS1 / blaLAP-2 / tet(A) / catA2 / sul2 / dfrA14	-	1..99066	99066

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T184225 WP_071177730.1 NZ_CP066252:53004-53318 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T184225 NZ_CP087571:c44858-44553 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTCTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT184225 WP_000093040.1 NZ_CP066252:52738-53016 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT184225 NZ_CP087571:c45078-44860 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATACAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1