Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 122297..122940 Replicon plasmid p2
Accession NZ_CP066250
Organism Klebsiella pneumoniae strain CRKP52R

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag JCR34_RS27640 Protein ID WP_001044770.1
Coordinates 122297..122713 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag JCR34_RS27645 Protein ID WP_001261282.1
Coordinates 122710..122940 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCR34_RS27620 118400..118672 - 273 Protein_132 transposase -
JCR34_RS27625 118701..119398 - 698 Protein_133 IS1 family transposase -
JCR34_RS27630 119654..120676 - 1023 WP_000361404.1 helicase UvrD -
JCR34_RS27635 120661..122223 - 1563 WP_004206609.1 AAA family ATPase -
JCR34_RS27640 122297..122713 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
JCR34_RS27645 122710..122940 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JCR34_RS27650 122897..123139 + 243 Protein_138 hypothetical protein -
JCR34_RS27655 123238..123528 + 291 WP_004213078.1 hypothetical protein -
JCR34_RS27660 123518..124417 + 900 WP_004225022.1 nucleotide-binding protein -
JCR34_RS27665 124466..126691 - 2226 WP_004215174.1 exclusion suppressor FxsA -
JCR34_RS27670 126693..127595 - 903 WP_004215173.1 hypothetical protein -
JCR34_RS27675 127679..127861 + 183 WP_004225020.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA2 / iutA / iucD / iucC / iucB / iucA 1..193221 193221
- inside IScluster/Tn - - 94055..119078 25023


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T184218 WP_001044770.1 NZ_CP066250:c122713-122297 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T184218 NZ_CP087570:3233251-3233505 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT184218 WP_001261282.1 NZ_CP066250:c122940-122710 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT184218 NZ_CP087570:3233003-3233254 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References