Detailed information of TA system TA184216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 56763..57673
• Replicon: plasmid p2
• Accession: NZ_CP066250
• Organism: Klebsiella pneumoniae strain CRKP52R

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: JCR34_RS27290
• Protein ID: WP_004026354.1
• Coordinates: 57203..57673 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: JCR34_RS27285
• Protein ID: WP_004026357.1
• Coordinates: 56763..57206 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCR34_RS27255	52074..52340	+	267	Protein_59	IS3 family transposase	-
JCR34_RS27260	52410..53063	-	654	WP_011251259.1	class I SAM-dependent methyltransferase	-
JCR34_RS27265	53399..54379	-	981	WP_000019473.1	IS5-like element ISKpn26 family transposase	-
JCR34_RS27270	54805..54903	+	99	Protein_62	response regulator	-
JCR34_RS27275	54973..55953	+	981	WP_000019473.1	IS5-like element ISKpn26 family transposase	-
JCR34_RS27280	56159..56662	+	504	Protein_64	DUF4113 domain-containing protein	-
JCR34_RS27285	56763..57206	+	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
JCR34_RS27290	57203..57673	+	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
JCR34_RS27295	57822..58519	+	698	Protein_67	IS1 family transposase	-
JCR34_RS27300	58536..58970	+	435	Protein_68	thioredoxin fold domain-containing protein	-
JCR34_RS27305	58987..59298	+	312	WP_011251282.1	hypothetical protein	-
JCR34_RS27310	59312..59653	+	342	WP_011251281.1	hypothetical protein	-
JCR34_RS27315	59713..60669	+	957	WP_011251280.1	DsbA family protein	-
JCR34_RS27320	61094..61534	-	441	WP_011251275.1	hypothetical protein	-
JCR34_RS27325	61524..62034	-	511	Protein_73	hypothetical protein	-
JCR34_RS27330	62043..62357	-	315	WP_011251273.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA	1..193221	193221
	-	inside	IScluster/Tn	-	-	48148..63954	15806

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T184216 WP_004026354.1 NZ_CP066250:57203-57673 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T184216 NZ_CP087570:2721208-2721606 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT184216 WP_004026357.1 NZ_CP066250:56763-57206 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT184216 NZ_CP087570:2720981-2721208 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9