Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 56763..57673 Replicon plasmid p2
Accession NZ_CP066250
Organism Klebsiella pneumoniae strain CRKP52R

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag JCR34_RS27290 Protein ID WP_004026354.1
Coordinates 57203..57673 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag JCR34_RS27285 Protein ID WP_004026357.1
Coordinates 56763..57206 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCR34_RS27255 52074..52340 + 267 Protein_59 IS3 family transposase -
JCR34_RS27260 52410..53063 - 654 WP_011251259.1 class I SAM-dependent methyltransferase -
JCR34_RS27265 53399..54379 - 981 WP_000019473.1 IS5-like element ISKpn26 family transposase -
JCR34_RS27270 54805..54903 + 99 Protein_62 response regulator -
JCR34_RS27275 54973..55953 + 981 WP_000019473.1 IS5-like element ISKpn26 family transposase -
JCR34_RS27280 56159..56662 + 504 Protein_64 DUF4113 domain-containing protein -
JCR34_RS27285 56763..57206 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
JCR34_RS27290 57203..57673 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
JCR34_RS27295 57822..58519 + 698 Protein_67 IS1 family transposase -
JCR34_RS27300 58536..58970 + 435 Protein_68 thioredoxin fold domain-containing protein -
JCR34_RS27305 58987..59298 + 312 WP_011251282.1 hypothetical protein -
JCR34_RS27310 59312..59653 + 342 WP_011251281.1 hypothetical protein -
JCR34_RS27315 59713..60669 + 957 WP_011251280.1 DsbA family protein -
JCR34_RS27320 61094..61534 - 441 WP_011251275.1 hypothetical protein -
JCR34_RS27325 61524..62034 - 511 Protein_73 hypothetical protein -
JCR34_RS27330 62043..62357 - 315 WP_011251273.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA2 / iutA / iucD / iucC / iucB / iucA 1..193221 193221
- inside IScluster/Tn - - 48148..63954 15806


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T184216 WP_004026354.1 NZ_CP066250:57203-57673 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T184216 NZ_CP087570:2721208-2721606 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT184216 WP_004026357.1 NZ_CP066250:56763-57206 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT184216 NZ_CP087570:2720981-2721208 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References