Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 471787..472424 Replicon chromosome
Accession NZ_CP066219
Organism Bacillus siamensis strain B28

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag JD965_RS02410 Protein ID WP_003156187.1
Coordinates 472074..472424 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag JD965_RS02405 Protein ID WP_003156188.1
Coordinates 471787..472068 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JD965_RS02385 468152..468751 - 600 WP_198698390.1 rhomboid family intramembrane serine protease -
JD965_RS02390 468844..469209 + 366 WP_198698391.1 holo-ACP synthase -
JD965_RS02395 469374..470381 + 1008 WP_016937172.1 outer membrane lipoprotein carrier protein LolA -
JD965_RS02400 470498..471667 + 1170 WP_016937171.1 alanine racemase -
JD965_RS02405 471787..472068 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
JD965_RS02410 472074..472424 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
JD965_RS02415 472545..473366 + 822 WP_003156182.1 STAS domain-containing protein -
JD965_RS02420 473371..473736 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
JD965_RS02425 473739..474140 + 402 WP_003156178.1 anti-sigma regulatory factor -
JD965_RS02430 474152..475159 + 1008 WP_016937170.1 PP2C family protein-serine/threonine phosphatase -
JD965_RS02435 475223..475552 + 330 WP_016937169.1 anti-sigma factor antagonist -
JD965_RS02440 475549..476031 + 483 WP_003156173.1 anti-sigma B factor RsbW -
JD965_RS02445 475997..476785 + 789 WP_016937168.1 RNA polymerase sigma factor SigB -
JD965_RS02450 476785..477387 + 603 WP_049627930.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T184178 WP_003156187.1 NZ_CP066219:472074-472424 [Bacillus siamensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T184178 NZ_CP087564:c368120-368013 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT184178 WP_003156188.1 NZ_CP066219:471787-472068 [Bacillus siamensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT184178 NZ_CP087564:368167-368233 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References