184171

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 497176..497747 Replicon chromosome
Accession NZ_CP066215
Organism Enterococcus faecium strain 18-042

Toxin (Protein)


Gene name mazF Uniprot ID A0A828ZXV4
Locus tag I8F50_RS02385 Protein ID WP_002302307.1
Coordinates 497406..497747 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag I8F50_RS02380 Protein ID WP_002323011.1
Coordinates 497176..497406 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8F50_RS02355 (I8F50_02350) 492530..493861 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
I8F50_RS02360 (I8F50_02355) 493883..494509 + 627 WP_002286816.1 cysteine hydrolase -
I8F50_RS02365 (I8F50_02360) 494702..495283 + 582 WP_002300332.1 TetR/AcrR family transcriptional regulator -
I8F50_RS02370 (I8F50_02365) 495770..496345 + 576 WP_002302305.1 SOS response-associated peptidase family protein -
I8F50_RS02375 (I8F50_02370) 496550..496888 - 339 WP_002286804.1 hypothetical protein -
I8F50_RS02380 (I8F50_02375) 497176..497406 + 231 WP_002323011.1 hypothetical protein Antitoxin
I8F50_RS02385 (I8F50_02380) 497406..497747 + 342 WP_002302307.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
I8F50_RS02390 (I8F50_02385) 497880..498788 + 909 WP_002295743.1 IS982-like element ISEfm1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13288.65 Da        Isoelectric Point: 9.9044

>T184171 WP_002302307.1 NZ_CP066215:497406-497747 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T184171 NZ_CP087392:c4286271-4285792 [Pectobacterium parvum]
GTGGGAATAACGGCACCTGAATTATTATTACCTCAGCATGCCGTGGTTGATTTCCGAAGTTCAGAACCGTCACTGGATGA
TTGGCTGAAACGTAAGGCGCTCAAGAATCAAACCATTGGCGCATCACGCACTTTTGTCGTATGTGAATCGGGTGCTAATC
AGGTTATTGGCTTTTACGCGCTGGCAACCGGCAGCGTACAGCGTCAATCCGTATCGGGTGCGTTACGACGCAATATGCCT
GATCCGCTGCCCGTATTAGTCTTGGGCCGTCTGGCGGTAGATGAACGTTATCACCGTCGGGGGATTGGCGCGGGACTATT
GAAAGATGCGGTCTTGCGTTCCCAGCAGGTTGCTGAACAGGTGGGAACAAAAGCGTTATTAGTTCATGCGCTGTCTGAGG
AAGCAAAAGATTTTTATCTGCATTGGGGCTTTACGTCCTCGGAAATCCAGCCTAGCACATTGCTTCTACCACTCTGGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT184171 WP_002323011.1 NZ_CP066215:497176-497406 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT184171 NZ_CP087392:c4286534-4286262 [Pectobacterium parvum]
ATGAGAACAGAGGCAAAAGAGGCACCGATCAACATTCGGGCGAAATCATCACAACGGGAACTGATAGACGTTGCCGCGAA
ACTGCTTTCAAAGTCACGTACTGATTTTATCCTTGAGGCTGCATGTCGTGAGGCGGAAGAGGTGCTATTGGATCAACGCC
TGTTTCTGGTTGATGAATCACAATTTGATGCCTTTATTACCGCGTTAGAAAAACCTGTCGCAACCAATGAGCGGTTACAG
GCGTTATTAGACAGGAAATCACCGTGGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXV4

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References