Detailed information of TA system TA184162

Bioinformatically predicted

Overview

TA module

• Type: III
• Classification (family/domain): toxIN/ToxN(toxin)
• Location: 52722..53452
• Replicon: plasmid pE81-84
• Accession: NZ_CP066196
• Organism: Bacillus cytotoxicus strain E8.1

Toxin (Protein)

• Gene name: toxN
• Uniprot ID: -
• Locus tag: JC776_RS21745
• Protein ID: WP_094374045.1
• Coordinates: 52880..53452 (+)
• Length: 191 a.a.

Antitoxin (RNA)

Gene name	toxI
Locus tag	-
Coordinates	52722..52818 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JC776_RS21720 (47830)	47830..48039	+	210	WP_094374055.1	DUF5511 family protein	-
JC776_RS21725 (48036)	48036..49805	+	1770	WP_162533114.1	ArdC-like ssDNA-binding domain-containing protein	-
JC776_RS21730 (49912)	49912..50892	+	981	WP_094374051.1	hypothetical protein	-
JC776_RS21735 (50917)	50917..51468	+	552	WP_094374049.1	metal-dependent hydrolase	-
JC776_RS21740 (51596)	51596..52618	+	1023	WP_094374047.1	DUF3991 and TOPRIM domain-containing protein	-
- (52722)	52722..52818	+	97	NuclAT_0	-	Antitoxin
- (52722)	52722..52818	+	97	NuclAT_0	-	Antitoxin
- (52722)	52722..52818	+	97	NuclAT_0	-	Antitoxin
- (52722)	52722..52818	+	97	NuclAT_0	-	Antitoxin
JC776_RS21745 (52880)	52880..53452	+	573	WP_094374045.1	type III toxin-antitoxin system ToxN/AbiQ family toxin	Toxin
JC776_RS21750 (53601)	53601..53981	+	381	WP_094374043.1	hypothetical protein	-
JC776_RS21755 (54353)	54353..55288	+	936	WP_094396514.1	hypothetical protein	-
JC776_RS21760 (55381)	55381..55962	+	582	Protein_63	toprim domain-containing protein	-
JC776_RS22435 (56089)	56089..56231	+	143	Protein_64	hypothetical protein	-
JC776_RS21765 (56284)	56284..56928	+	645	WP_239431074.1	TnsA endonuclease N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..83570	83570

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ToxN/AbiQ toxin (8-171)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 22516.91 Da
• Isoelectric Point: 9.5095

>T184162 WP_094374045.1 NZ_CP066196:52880-53452 [Bacillus cytotoxicus]
MANKDNPKFYTISTKYIDYLRETDNKVPFNKDEQHSRPYVGVLEKINGHDYFVPLTSRNDKNFNSQVSVKLFDKDNPEKR
IGVLLVNNMIPVPEKECKEIDIAEKMETDPKYGNLMLKQYLFLKENMDRLSHKVEKVYKDVTVQGKPSKKQKFLKGICCD
FSTLEEKCQEYKEKNQARRIAYMRQMGRER

Nucleotide

• Length: 573 bp

>T184162 NZ_CP087392:823233-823646 [Pectobacterium parvum]
GTGGCCCAGTGGCAATGTGACCTTCGTGTTTCCTGGCGCATGCAACTGCTGTCATTGGTTGTGCATGGCCTATTAGTATT
GCTCATTCTGCTGGCACCGTGGCCGGACGGCTATGCATGGCTATGGCTATGCCTGGTCACGATGGTGATGTTCGGTTTTA
TCCGTAGCCAAAAGAATATCAAATCCCGACAGGGGGAAATCGTTCTGCTTAGCGAAACGACCCTGAACTGGCGGCAGCAG
GAATGGCAGATCGTTAAACGACCGTGGCTGCTGAAGAATGGCGTATTGCTGTCATTGCGAGCGGTTAACGGTAAAGACAA
GCAACAGCTGTGGCTGGCATCGGACAGCATGGGTGATGAAGAGTGGCGTCACTTGCGTCAGCTTCTTTTGCAGCAGAAAC
ACTGGGCAAGATAG

Antitoxin

Nucleotide

• Length: 97 bp

>AT184162 NZ_CP066196:52722-52818 [Bacillus cytotoxicus]
GTAGCCTTACTTTAGTAGGCGCGAAAAAATTGGTGTAACTCTACCGTAGTGAGTACCAAAAAATTGGTGTAGCCTTACCG
TAGTAGGCACAAAAAAA

Structures

Antitoxin