Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 318124..318766 Replicon chromosome
Accession NZ_CP066192
Organism Bacillus cytotoxicus strain E17.4

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag JC775_RS01690 Protein ID WP_000635963.1
Coordinates 318416..318766 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8PTJ6
Locus tag JC775_RS01685 Protein ID WP_000004571.1
Coordinates 318124..318411 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JC775_RS01665 (JC775_01665) 314503..315063 - 561 WP_048721301.1 rhomboid family intramembrane serine protease -
JC775_RS01670 (JC775_01670) 315154..315513 + 360 WP_011983363.1 holo-ACP synthase -
JC775_RS01675 (JC775_01675) 315669..316619 + 951 WP_041810183.1 outer membrane lipoprotein carrier protein LolA -
JC775_RS01680 (JC775_01680) 316741..317910 + 1170 WP_011983365.1 alanine racemase -
JC775_RS01685 (JC775_01685) 318124..318411 + 288 WP_000004571.1 antitoxin EndoAI Antitoxin
JC775_RS01690 (JC775_01690) 318416..318766 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
JC775_RS01695 (JC775_01695) 318831..320999 + 2169 WP_048721313.1 Tex family protein -
JC775_RS01700 (JC775_01700) 321073..321207 - 135 WP_087094358.1 cortex morphogenetic protein CmpA -
JC775_RS01705 (JC775_01705) 321594..322058 + 465 WP_011983367.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T184158 WP_000635963.1 NZ_CP066192:318416-318766 [Bacillus cytotoxicus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T184158 NZ_CP087392:c30360-29989 [Pectobacterium parvum]
TTGGCATGGGAAATAGTCACACGGGCGCTATTCGATACTTGGTTTGATGAACAAACCGAAGCGGTACAGGAAGAGATTTT
GGCACACCTCGCTATACTCGAAGAGGATGGCCCACAGCTAGGACGACCACAAGTTGATCAGATTAAAGGCTCAAAATACA
AAAACATGAAGGAGCTTCGGGTTCAGGTTGGCGGCCACCCATTTCGAACATTCTTCGCGTTTGACCGGACACGTAAGGCG
ATTATTTTATGTGCGGGAGATAAGAAAGGCGAAGATGAAAAGTTGTTCTACGAACGTATGATTAAGATCGCAGACGCTGA
GTTTACATCGCATCTGATATCACCGGAGGTAAAAGAAGATGGCAACGCTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10748.30 Da        Isoelectric Point: 8.9605

>AT184158 WP_000004571.1 NZ_CP066192:318124-318411 [Bacillus cytotoxicus]
VSESSVTTEIVVRLPKQVVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT184158 NZ_CP087392:c30002-29703 [Pectobacterium parvum]
ATGGCAACGCTTAAAGAAATGATGGCTAAACGTTCACCGGAGAGCCGCGCCCGCATTGAAGCGCGCACGGCTGAGATCCG
TCAGGAAATCACTTTAGCGAAACTACGCGAAGAGCTGAACATGTCGCAAACTCAGCTTGCGCTAGCATTGGGCGTCAAGC
AGCCATCCATTGTTAAGATGGAGAAGGTAGAGAATGACCCAAAACTATCAACGCTGAAACGCTATGTCACCGCCCTAGGC
GGCAAGCTTACGCTAGATGTGACCTTGCCTGATGGCAAGCGTATCGCGCTTACTTTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB R8PTJ6

References