Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 1757703..1758345 Replicon chromosome
Accession NZ_CP066187
Organism Bacillus cytotoxicus strain E28.3

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag JC772_RS09205 Protein ID WP_000635963.1
Coordinates 1757995..1758345 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8PTJ6
Locus tag JC772_RS09200 Protein ID WP_000004571.1
Coordinates 1757703..1757990 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JC772_RS09180 (JC772_09180) 1754082..1754642 - 561 WP_048721301.1 rhomboid family intramembrane serine protease -
JC772_RS09185 (JC772_09185) 1754733..1755092 + 360 WP_011983363.1 holo-ACP synthase -
JC772_RS09190 (JC772_09190) 1755248..1756198 + 951 WP_041810183.1 outer membrane lipoprotein carrier protein LolA -
JC772_RS09195 (JC772_09195) 1756320..1757489 + 1170 WP_011983365.1 alanine racemase -
JC772_RS09200 (JC772_09200) 1757703..1757990 + 288 WP_000004571.1 antitoxin EndoAI Antitoxin
JC772_RS09205 (JC772_09205) 1757995..1758345 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
JC772_RS09210 (JC772_09210) 1758410..1760578 + 2169 WP_048721313.1 Tex family protein -
JC772_RS09215 (JC772_09215) 1760652..1760786 - 135 WP_087094358.1 cortex morphogenetic protein CmpA -
JC772_RS09220 (JC772_09220) 1761173..1761637 + 465 WP_011983367.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T184152 WP_000635963.1 NZ_CP066187:1757995-1758345 [Bacillus cytotoxicus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T184152 NZ_CP087390:2873568-2873855 [Enterobacter sp. AN-K1]
ATGCCTATAGAGTATGAGTGGGATAGCAATAAGGCGAAAAGCAATCTGCAAAAACACGCCATTCGTTTTGAAGATGCAGT
TCTGGTTTTTGACGATCCCTGCCATTTGTCTGTTCAGGATCGTTATGAAAACGGGGAGTTTCGCTGGCAAACCATTGGTC
TTGTACAGGGTGTTATTGTCATTCTGGTCGCGCACACGGTATGTTTTGAAAGCGGGGATGAAATCATACGTATCATCAGT
GCGCGTAAAGCGGATCGTAAAGAGAGGAGGCGTTATGAGCATCGTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10748.30 Da        Isoelectric Point: 8.9605

>AT184152 WP_000004571.1 NZ_CP066187:1757703-1757990 [Bacillus cytotoxicus]
VSESSVTTEIVVRLPKQVVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT184152 NZ_CP087390:2873842-2874144 [Enterobacter sp. AN-K1]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCACCGGAAGTGAGTGCCCGCCGGGAGGCTGAACTCAAAGCGCTGGCGGA
TAAACCCGACAGGGAAATTGATTACAGCGATATACCGGCCACGGAGGATGATCGCTGGGCCGGTGCCGTGCGGGGAAAAT
TCTATCGACCGCTCAAAACTCAGGCATCAGTACGCATTGACGCCGACGTGATGGAGTGGCTTAAACGGCCAGGGAAAGGT
TACCAGACGCGGCTTAATGCCATTTTGCGCGAGGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB R8PTJ6

References