Detailed information of TA system TA184151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 3094949..3095941
• Replicon: chromosome
• Accession: NZ_CP066185
• Organism: Bacillus cytotoxicus strain PDT2.12

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A7GPN6
• Locus tag: JC773_RS15685
• Protein ID: WP_012094283.1
• Coordinates: 3094949..3095689 (+)
• Length: 247 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A7GPN7
• Locus tag: JC773_RS15690
• Protein ID: WP_012094285.1
• Coordinates: 3095813..3095941 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JC773_RS15660 (JC773_15660)	3090422..3091324	+	903	WP_210351571.1	BadF/BadG/BcrA/BcrD ATPase family protein	-
JC773_RS15665 (JC773_15665)	3091348..3091677	-	330	Protein_3024	hypothetical protein	-
JC773_RS15670 (JC773_15670)	3091873..3093177	+	1305	WP_087098711.1	DEAD/DEAH box helicase	-
JC773_RS15675 (JC773_15675)	3093397..3093879	+	483	WP_012094281.1	helix-turn-helix domain-containing protein	-
JC773_RS15680 (JC773_15680)	3094017..3094754	+	738	WP_012094282.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
JC773_RS15685 (JC773_15685)	3094949..3095689	+	741	WP_012094283.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
JC773_RS15690 (JC773_15690)	3095813..3095941	+	129	WP_012094285.1	hypothetical protein	Antitoxin
JC773_RS15695 (JC773_15695)	3096018..3096194	+	177	WP_087094925.1	stage II sporulation protein SB	-
JC773_RS15700 (JC773_15700)	3096353..3096898	-	546	WP_087094926.1	GNAT family N-acetyltransferase	-
JC773_RS15705 (JC773_15705)	3096939..3097820	-	882	WP_012094288.1	aminoglycoside 6-adenylyltransferase	-
JC773_RS15710 (JC773_15710)	3098026..3099495	+	1470	WP_012094289.1	aminoacyl-histidine dipeptidase	-
JC773_RS15715 (JC773_15715)	3100085..3100336	+	252	WP_012094290.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-244)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 247 a.a.
• Molecular weight: 28628.30 Da
• Isoelectric Point: 6.7302

>T184151 WP_012094283.1 NZ_CP066185:3094949-3095689 [Bacillus cytotoxicus]
MTVTSVRIGLFFLVIFFLFLIFFYWRNEERYEERKQLIRKTWYGLFITSVTMYFMMKGIDLTLWKHILMVVAMVVFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTLIQNVNPAAFGTMEWETDAEYARSLNAFLDTYAEKIGAK
IVVFVTEEELNTNFRGVRSQFSITVSPESIEQLNEQKAVQIENVGIIPAKIIKDVYIIMDGQKNNLQDRDFENVYNLTIH
HSYFCK

Nucleotide

• Length: 741 bp

>T184151 NZ_CP087390:2281945-2282259 [Enterobacter sp. AN-K1]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAACACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4984.43 Da
• Isoelectric Point: 3.9944

>AT184151 WP_012094285.1 NZ_CP066185:3095813-3095941 [Bacillus cytotoxicus]
VLKEYSNDFFKDEAEDTTDFSLIKGDTMQQVEELEEELKRRG

Nucleotide

• Length: 129 bp

>AT184151 NZ_CP087390:2282256-2282675 [Enterobacter sp. AN-K1]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCACGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGCCCCTTGCTGGATATTGTGT
GCGCCAGAATTAGCCATTTCGAAGCTAATCGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTTGGA
ATTGCAGTTTTAAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGAT
GGTTTCACGGGTTCTGAATGGTCAGAGACAGCTTACTCTGAACCATATCAAAAAGCTGGCGGCCAGATTTGGGGTATCAC
CTGCGTTATTTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A7GPN6

Antitoxin

• AlphaFold DB: A7GPN7