Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 1396449..1397091 Replicon chromosome
Accession NZ_CP066185
Organism Bacillus cytotoxicus strain PDT2.12

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag JC773_RS07315 Protein ID WP_000635963.1
Coordinates 1396741..1397091 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8PTJ6
Locus tag JC773_RS07310 Protein ID WP_000004571.1
Coordinates 1396449..1396736 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JC773_RS07290 (JC773_07290) 1392829..1393389 - 561 WP_011983362.1 rhomboid family intramembrane serine protease -
JC773_RS07295 (JC773_07295) 1393480..1393839 + 360 WP_011983363.1 holo-ACP synthase -
JC773_RS07300 (JC773_07300) 1393995..1394945 + 951 WP_041810183.1 outer membrane lipoprotein carrier protein LolA -
JC773_RS07305 (JC773_07305) 1395066..1396235 + 1170 WP_011983365.1 alanine racemase -
JC773_RS07310 (JC773_07310) 1396449..1396736 + 288 WP_000004571.1 antitoxin EndoAI Antitoxin
JC773_RS07315 (JC773_07315) 1396741..1397091 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
JC773_RS07320 (JC773_07320) 1397156..1399324 + 2169 WP_011983366.1 Tex family protein -
JC773_RS07325 (JC773_07325) 1399398..1399532 - 135 WP_087097560.1 cortex morphogenetic protein CmpA -
JC773_RS07330 (JC773_07330) 1399911..1400375 + 465 WP_011983367.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T184150 WP_000635963.1 NZ_CP066185:1396741-1397091 [Bacillus cytotoxicus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T184150 NZ_CP087390:c2162216-2161845 [Enterobacter sp. AN-K1]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGTGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10748.30 Da        Isoelectric Point: 8.9605

>AT184150 WP_000004571.1 NZ_CP066185:1396449-1396736 [Bacillus cytotoxicus]
VSESSVTTEIVVRLPKQVVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT184150 NZ_CP087390:c2162461-2162219 [Enterobacter sp. AN-K1]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAACTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB R8PTJ6

References