Detailed information of TA system TA184149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 3152412..3153404
• Replicon: chromosome
• Accession: NZ_CP066184
• Organism: Bacillus cytotoxicus strain SM1.1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A7GPN6
• Locus tag: JC777_RS17085
• Protein ID: WP_012094283.1
• Coordinates: 3152664..3153404 (-)
• Length: 247 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A7GPN7
• Locus tag: JC777_RS17080
• Protein ID: WP_012094285.1
• Coordinates: 3152412..3152540 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JC777_RS17055 (JC777_17060)	3148017..3148268	-	252	WP_012094290.1	hypothetical protein	-
JC777_RS17060 (JC777_17065)	3148858..3150327	-	1470	WP_012094289.1	aminoacyl-histidine dipeptidase	-
JC777_RS17065 (JC777_17070)	3150533..3151414	+	882	WP_012094288.1	aminoglycoside 6-adenylyltransferase	-
JC777_RS17070 (JC777_17075)	3151455..3152000	+	546	WP_087094926.1	GNAT family N-acetyltransferase	-
JC777_RS17075 (JC777_17080)	3152159..3152335	-	177	WP_087094925.1	stage II sporulation protein SB	-
JC777_RS17080 (JC777_17085)	3152412..3152540	-	129	WP_012094285.1	hypothetical protein	Antitoxin
JC777_RS17085 (JC777_17090)	3152664..3153404	-	741	WP_012094283.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
JC777_RS17090 (JC777_17095)	3153599..3154336	-	738	WP_012094282.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
JC777_RS17095 (JC777_17100)	3154474..3154956	-	483	WP_012094281.1	helix-turn-helix domain-containing protein	-
JC777_RS17100 (JC777_17105)	3155176..3156480	-	1305	WP_087098711.1	DEAD/DEAH box helicase	-
JC777_RS17105 (JC777_17110)	3156676..3157005	+	330	Protein_3154	hypothetical protein	-
JC777_RS17110 (JC777_17115)	3157029..3157931	-	903	WP_087098709.1	BadF/BadG/BcrA/BcrD ATPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-244)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 247 a.a.
• Molecular weight: 28628.30 Da
• Isoelectric Point: 6.7302

>T184149 WP_012094283.1 NZ_CP066184:c3153404-3152664 [Bacillus cytotoxicus]
MTVTSVRIGLFFLVIFFLFLIFFYWRNEERYEERKQLIRKTWYGLFITSVTMYFMMKGIDLTLWKHILMVVAMVVFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTLIQNVNPAAFGTMEWETDAEYARSLNAFLDTYAEKIGAK
IVVFVTEEELNTNFRGVRSQFSITVSPESIEQLNEQKAVQIENVGIIPAKIIKDVYIIMDGQKNNLQDRDFENVYNLTIH
HSYFCK

Nucleotide

• Length: 741 bp

>T184149 NZ_CP087390:c1378052-1377642 [Enterobacter sp. AN-K1]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGGCTGGTGGCGGC
ACTGGTGTTATTGGTGCCGTGGCCGCTTAGCTATACCCCTCTGTGGTTGCTATTGCTGTCGTTTGTGGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAGATGATGGGGACGCCGTGGATGCTTAATACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGATAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCTGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4984.43 Da
• Isoelectric Point: 3.9944

>AT184149 WP_012094285.1 NZ_CP066184:c3152540-3152412 [Bacillus cytotoxicus]
VLKEYSNDFFKDEAEDTTDFSLIKGDTMQQVEELEEELKRRG

Nucleotide

• Length: 129 bp

>AT184149 NZ_CP087390:c1378299-1378033 [Enterobacter sp. AN-K1]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A7GPN6

Antitoxin

• AlphaFold DB: A7GPN7