Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 670419..671061 Replicon chromosome
Accession NZ_CP066184
Organism Bacillus cytotoxicus strain SM1.1

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag JC777_RS04110 Protein ID WP_000635963.1
Coordinates 670419..670769 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8PTJ6
Locus tag JC777_RS04115 Protein ID WP_000004571.1
Coordinates 670774..671061 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JC777_RS04095 (JC777_04095) 667135..667599 - 465 WP_011983367.1 SprT family protein -
JC777_RS04100 (JC777_04100) 667978..668112 + 135 WP_087097560.1 cortex morphogenetic protein CmpA -
JC777_RS04105 (JC777_04105) 668186..670354 - 2169 WP_011983366.1 Tex family protein -
JC777_RS04110 (JC777_04110) 670419..670769 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
JC777_RS04115 (JC777_04115) 670774..671061 - 288 WP_000004571.1 antitoxin EndoAI Antitoxin
JC777_RS04120 (JC777_04120) 671275..672444 - 1170 WP_011983365.1 alanine racemase -
JC777_RS04125 (JC777_04125) 672566..673516 - 951 WP_041810183.1 outer membrane lipoprotein carrier protein LolA -
JC777_RS04130 (JC777_04130) 673672..674031 - 360 WP_011983363.1 holo-ACP synthase -
JC777_RS04135 (JC777_04135) 674122..674682 + 561 WP_048721301.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T184148 WP_000635963.1 NZ_CP066184:c670769-670419 [Bacillus cytotoxicus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T184148 NZ_CP087390:1151507-1151806 [Enterobacter sp. AN-K1]
ATGATTAATCATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATCCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10748.30 Da        Isoelectric Point: 8.9605

>AT184148 WP_000004571.1 NZ_CP066184:c671061-670774 [Bacillus cytotoxicus]
VSESSVTTEIVVRLPKQVVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT184148 NZ_CP087390:1151817-1152182 [Enterobacter sp. AN-K1]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAATATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAACTTGGCAAGCCAGACGTTACCCTGCACGGGAACAGCGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB R8PTJ6

References