Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | pemIK/MazF(toxin) |
| Location | 443869..444511 | Replicon | chromosome |
| Accession | NZ_CP066179 | ||
| Organism | Bacillus cytotoxicus strain SM2.8 | ||
Toxin (Protein)
| Gene name | pemK | Uniprot ID | J8CWW5 |
| Locus tag | JC774_RS02300 | Protein ID | WP_000635963.1 |
| Coordinates | 443869..444219 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | pemI | Uniprot ID | R8PTJ6 |
| Locus tag | JC774_RS02305 | Protein ID | WP_000004571.1 |
| Coordinates | 444224..444511 (-) | Length | 96 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JC774_RS02285 (JC774_02285) | 440585..441049 | - | 465 | WP_011983367.1 | SprT family protein | - |
| JC774_RS02290 (JC774_02290) | 441428..441562 | + | 135 | WP_087097560.1 | cortex morphogenetic protein CmpA | - |
| JC774_RS02295 (JC774_02295) | 441636..443804 | - | 2169 | WP_011983366.1 | Tex family protein | - |
| JC774_RS02300 (JC774_02300) | 443869..444219 | - | 351 | WP_000635963.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| JC774_RS02305 (JC774_02305) | 444224..444511 | - | 288 | WP_000004571.1 | antitoxin EndoAI | Antitoxin |
| JC774_RS02310 (JC774_02310) | 444725..445894 | - | 1170 | WP_011983365.1 | alanine racemase | - |
| JC774_RS02315 (JC774_02315) | 446016..446966 | - | 951 | WP_041810183.1 | outer membrane lipoprotein carrier protein LolA | - |
| JC774_RS02320 (JC774_02320) | 447122..447481 | - | 360 | WP_011983363.1 | holo-ACP synthase | - |
| JC774_RS02325 (JC774_02325) | 447572..448132 | + | 561 | WP_048721301.1 | rhomboid family intramembrane serine protease | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12974.12 Da Isoelectric Point: 5.7168
>T184146 WP_000635963.1 NZ_CP066179:c444219-443869 [Bacillus cytotoxicus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF
Download Length: 351 bp
>T184146 NZ_CP087390:c264154-264051 [Enterobacter sp. AN-K1]
GGCAAGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT
GGCAAGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT
Antitoxin
Download Length: 96 a.a. Molecular weight: 10748.30 Da Isoelectric Point: 8.9605
>AT184146 WP_000004571.1 NZ_CP066179:c444511-444224 [Bacillus cytotoxicus]
VSESSVTTEIVVRLPKQVVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG
VSESSVTTEIVVRLPKQVVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG
Download Length: 288 bp
>AT184146 NZ_CP087390:264046-264247 [Enterobacter sp. AN-K1]
CAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTCAATCGCCTTGCCCTTTAAGAATAGATGACGACGTCAGGTTTTCCAGTCCACAGTAAAAGTGGT
CTGAAAAAAAGCGTCAGAACATCACTAAATGTGAAAAACCGC
CAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTCAATCGCCTTGCCCTTTAAGAATAGATGACGACGTCAGGTTTTCCAGTCCACAGTAAAAGTGGT
CTGAAAAAAAGCGTCAGAACATCACTAAATGTGAAAAACCGC
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|