Detailed information of TA system TA184144

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 144238..144908
• Replicon: plasmid pKP2011790
• Accession: NZ_CP066174
• Organism: Klebsiella quasipneumoniae strain KP2011790

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: JDZ43_RS25005
• Protein ID: WP_004213072.1
• Coordinates: 144238..144681 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: JDZ43_RS25010
• Protein ID: WP_004213073.1
• Coordinates: 144678..144908 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JDZ43_RS24970	139648..139923	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
JDZ43_RS24975	139986..140477	+	492	WP_011251327.1	DM13 domain-containing protein	-
JDZ43_RS24980	140526..141446	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
JDZ43_RS24985	141537..141943	+	407	Protein_146	GAF domain-containing protein	-
JDZ43_RS24990	142458..143094	-	637	Protein_147	mucoid phenotype A regulator RmpA	-
JDZ43_RS24995	143511..143804	+	294	Protein_148	transposase	-
JDZ43_RS25000	143808..144224	+	417	Protein_149	restriction endonuclease	-
JDZ43_RS25005	144238..144681	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
JDZ43_RS25010	144678..144908	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JDZ43_RS25015	145516..146649	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
JDZ43_RS25020	146665..146958	+	294	WP_004213076.1	hypothetical protein	-
JDZ43_RS25025	146948..147154	-	207	WP_004213077.1	hypothetical protein	-
JDZ43_RS25030	147506..147796	+	291	WP_004213078.1	hypothetical protein	-
JDZ43_RS25035	147786..148685	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..210864	210864

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T184144 WP_004213072.1 NZ_CP066174:c144681-144238 [Klebsiella quasipneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T184144 NZ_CP087381:842750-843034 [Desulfovibrio subterraneus]
ATGCCGCCAACCTTCAAATGCAAAAAGACGCAGGCACTGTACGAGACAGGGGCTTCCCGCCAGTTTCAAGCAGTTATCAA
GGTTGCGTTACGAAAACTGGATATGATTTTTGCTGCCGCTGTGCTGGAAGATTTACGCATCCCGCCGGCGAACAGGCTGG
AAGCACTGAAAGGTGACAGAGCCGGACAGCACAGCATCCGCATAAACGACCAATGGCGCATTTGCTTTGAGTGGGACACC
GCCCAAAACCGCGCCAGCAACATAGAAATAGTTGATTATCACTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT184144 WP_004213073.1 NZ_CP066174:c144908-144678 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT184144 NZ_CP087381:843052-843351 [Desulfovibrio subterraneus]
ATGCGTATCAAAACCCACCCCGGCGAGGTCTTGCGCGAGGAATTCATGGTTCCCTACAAGCTTTCGGCCAGCAAGCTTTC
CGGCTATCTGGGGGTGCCTCTTTCGCGCATTACCGAGATTGCCGCTGAAAAGCGCGGCATCACAGCCGACACCGCAGTGC
GCCTTGCCAAGTTCTTCGGCACCACGCCGGAATTCTGGACCAACCTGCAGACGCAGCACGACCTTTCCAAGGTGCAGAAT
ACAAAAACGCCCGAAATCGGGCGCATTCCACGGTGTCAGGAAGTGGCTGCCTGTTTCTAG

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620