Detailed information of TA system TA184142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 51585..52321
• Replicon: plasmid pKP2011790
• Accession: NZ_CP066174
• Organism: Klebsiella quasipneumoniae strain KP2011790

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: JDZ43_RS24500
• Protein ID: WP_117985291.1
• Coordinates: 51839..52321 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A7D3T1D0
• Locus tag: JDZ43_RS24495
• Protein ID: WP_004213599.1
• Coordinates: 51585..51851 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JDZ43_RS24470	46817..47374	+	558	WP_004213590.1	recombinase family protein	-
JDZ43_RS24475	47377..50346	+	2970	WP_004213592.1	Tn3 family transposase	-
JDZ43_RS24480	50388..50882	+	495	WP_004213594.1	hypothetical protein	-
JDZ43_RS24485	50943..51146	+	204	WP_004213596.1	hemolysin expression modulator Hha	-
JDZ43_RS24490	51160..51390	+	231	WP_004213598.1	hypothetical protein	-
JDZ43_RS24495	51585..51851	+	267	WP_004213599.1	DUF1778 domain-containing protein	Antitoxin
JDZ43_RS24500	51839..52321	+	483	WP_117985291.1	GNAT family N-acetyltransferase	Toxin
JDZ43_RS24505	52742..53969	+	1228	Protein_50	IS3 family transposase	-
JDZ43_RS24510	53965..54360	-	396	Protein_51	DDE-type integrase/transposase/recombinase	-
JDZ43_RS24515	54535..55557	-	1023	WP_004214536.1	porphobilinogen synthase	-
JDZ43_RS24520	55621..56967	-	1347	WP_048333011.1	dihydroorotase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..210864	210864
	-	inside	IScluster/Tn	-	-	46817..53969	7152

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17335.06 Da
• Isoelectric Point: 10.0704

>T184142 WP_117985291.1 NZ_CP066174:51839-52321 [Klebsiella quasipneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENLGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T184142 NZ_CP087379:1976187-1976363 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10151.58 Da
• Isoelectric Point: 4.5141

>AT184142 WP_004213599.1 NZ_CP066174:51585-51851 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT184142 NZ_CP087379:1976419-1976835 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Antitoxin

• AlphaFold DB: A0A7D3T1D0