Detailed information of TA system TA184118

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 4869..5449
• Replicon: plasmid unnamed4
• Accession: NZ_CP066155
• Organism: Klebsiella pneumoniae strain KP697

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: JDZ44_RS29620
• Protein ID: WP_071177730.1
• Coordinates: 4869..5183 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: JDZ44_RS29625
• Protein ID: WP_000093040.1
• Coordinates: 5171..5449 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JDZ44_RS29595 (JDZ44_29485)	813..2777	+	1965	WP_071177727.1	TraM recognition domain-containing protein	-
JDZ44_RS29600 (JDZ44_29490)	2777..3508	+	732	WP_071177728.1	MobC family replication-relaxation protein	-
JDZ44_RS29605 (JDZ44_29495)	3515..4045	+	531	WP_071177729.1	hypothetical protein	-
JDZ44_RS29610 (JDZ44_29500)	4072..4251	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
JDZ44_RS29615 (JDZ44_29505)	4277..4705	-	429	WP_001140599.1	hypothetical protein	-
JDZ44_RS29620 (JDZ44_29510)	4869..5183	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JDZ44_RS29625 (JDZ44_29515)	5171..5449	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
JDZ44_RS29630	5624..5989	-	366	WP_072354022.1	TonB family protein	-
JDZ44_RS29635 (JDZ44_29525)	5986..6357	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
JDZ44_RS29640 (JDZ44_29530)	6631..6876	-	246	WP_032440458.1	hypothetical protein	-
JDZ44_RS29645 (JDZ44_29535)	7521..9008	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11970	11970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T184118 WP_071177730.1 NZ_CP066155:c5183-4869 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T184118 NZ_CP087377:1083608-1084015 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT184118 WP_000093040.1 NZ_CP066155:c5449-5171 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT184118 NZ_CP087377:1083361-1083627 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGATGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1