Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 124157..124827 Replicon plasmid unnamed1
Accession NZ_CP066152
Organism Klebsiella pneumoniae strain KP697

Toxin (Protein)


Gene name vagD Uniprot ID Q6U619
Locus tag JDZ44_RS27850 Protein ID WP_004213072.1
Coordinates 124157..124600 (-) Length 148 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q6U620
Locus tag JDZ44_RS27855 Protein ID WP_004213073.1
Coordinates 124597..124827 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JDZ44_RS27815 (JDZ44_27735) 119568..119843 + 276 WP_004213932.1 carboxymuconolactone decarboxylase family protein -
JDZ44_RS27820 (JDZ44_27740) 119906..120397 + 492 WP_011251327.1 DM13 domain-containing protein -
JDZ44_RS27825 (JDZ44_27745) 120446..121366 + 921 WP_004213934.1 DUF1471 domain-containing protein -
JDZ44_RS27830 (JDZ44_27750) 121457..121860 + 404 Protein_140 GAF domain-containing protein -
JDZ44_RS27835 (JDZ44_27755) 122378..123013 - 636 Protein_141 mucoid phenotype regulator RmpA2 -
JDZ44_RS27840 (JDZ44_27760) 123430..123734 + 305 Protein_142 transposase -
JDZ44_RS27845 123757..124008 - 252 WP_186987481.1 hypothetical protein -
JDZ44_RS27850 (JDZ44_27770) 124157..124600 - 444 WP_004213072.1 type II toxin-antitoxin system VapC family toxin Toxin
JDZ44_RS27855 (JDZ44_27775) 124597..124827 - 231 WP_004213073.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JDZ44_RS27860 (JDZ44_27780) 125435..126568 + 1134 WP_004213075.1 DUF3800 domain-containing protein -
JDZ44_RS27865 (JDZ44_27785) 126584..126877 + 294 WP_004213076.1 hypothetical protein -
JDZ44_RS27870 (JDZ44_27790) 126867..127073 - 207 WP_004213077.1 hypothetical protein -
JDZ44_RS27875 (JDZ44_27795) 127425..127715 + 291 WP_004213078.1 hypothetical protein -
JDZ44_RS27880 (JDZ44_27800) 127705..128604 + 900 WP_004225022.1 nucleotide-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..197408 197408


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16006.74 Da        Isoelectric Point: 9.3642

>T184112 WP_004213072.1 NZ_CP066152:c124600-124157 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Download         Length: 444 bp

>T184112 NZ_CP087377:262336-262443 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.73 Da        Isoelectric Point: 4.4563

>AT184112 WP_004213073.1 NZ_CP066152:c124827-124597 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT184112 NZ_CP087377:c262278-262224 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q6U619

Antitoxin

Source ID Structure
AlphaFold DB Q6U620

References