Detailed information of TA system TA184086

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2051704..2052785
• Replicon: chromosome
• Accession: NZ_CP066149
• Organism: Vibrio sp. ED004

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: ITG10_RS09300
• Protein ID: WP_248386367.1
• Coordinates: 2051704..2052267 (-)
• Length: 188 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: ITG10_RS09305
• Protein ID: WP_017632888.1
• Coordinates: 2052333..2052785 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG10_RS09275 (ITG10_09185)	2047017..2048543	-	1527	WP_248386365.1	IS66 family transposase	-
ITG10_RS09280 (ITG10_09190)	2048603..2048956	-	354	WP_016791133.1	IS66 family insertion sequence element accessory protein TnpB	-
ITG10_RS09285 (ITG10_09195)	2048953..2049264	-	312	WP_016768965.1	hypothetical protein	-
ITG10_RS09290 (ITG10_09200)	2049398..2049520	+	123	Protein_1775	IS5/IS1182 family transposase	-
ITG10_RS09295 (ITG10_09205)	2049615..2051168	-	1554	WP_248386366.1	response regulator	-
ITG10_RS09300 (ITG10_09210)	2051704..2052267	-	564	WP_248386367.1	PIN domain-containing protein	Toxin
ITG10_RS09305 (ITG10_09215)	2052333..2052785	-	453	WP_017632888.1	excisionase family DNA-binding protein	Antitoxin
ITG10_RS09310 (ITG10_09220)	2053254..2053493	+	240	WP_017632887.1	hypothetical protein	-
ITG10_RS09315 (ITG10_09225)	2053661..2053954	+	294	WP_248386368.1	hypothetical protein	-
ITG10_RS09320 (ITG10_09240)	2054930..2056285	+	1356	WP_248386369.1	Fic family protein	-
ITG10_RS09325 (ITG10_09245)	2056399..2056911	-	513	Protein_1782	transcriptional regulator	-
ITG10_RS09330 (ITG10_09250)	2056905..2057176	-	272	Protein_1783	DUF4160 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2048953..2063971	15018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-91)

Antitoxin

• SinI-like, DNA-binding domain (80-126)

Sequences

Toxin

Protein

• Length: 188 a.a.
• Molecular weight: 21429.45 Da
• Isoelectric Point: 6.3337

>T184086 WP_248386367.1 NZ_CP066149:c2052267-2051704 [Vibrio sp. ED004]
MHLANTGLFRARWTEQIHDEWTRNLLKNRPELNTEQLQRTRQLMNANVPDCLIEGYEPLIDGLTLPDVNDRHVVAAAIKG
QAESIITFNLKDFPSSELEPLGLSAIHPDEFLYDMFELDNSACVKAAQQQRASLKRPPMSQDEFLAYLQKQRLPSFVSHL
KSFKLQVDALDLICSNKCRRLDKNQPA

Nucleotide

• Length: 564 bp

>T184086 NZ_CP087331:639025-639414 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCAATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AATTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCACGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGTAGATTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16449.85 Da
• Isoelectric Point: 4.6723

>AT184086 WP_017632888.1 NZ_CP066149:c2052785-2052333 [Vibrio sp. ED004]
MNIATKLPSAEEMALAKLGSQELSAVIEINGEAQRINVVDKSGKTHEITIPVSALNMMIEVLTQLGQGNSVSITPIHAEL
TTQEGADMLSMSRPTFIKLLDSGDIPFSRTGNRRKVAYADLMEYKHRLEENKLTALAELSALDQEMDMGY

Nucleotide

• Length: 453 bp

>AT184086 NZ_CP087331:638761-639018 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA